NYIMAS ROSDIANI AMINAH, 051211131017 (2016) KARAKTERISASI MIKROSFER NISIN DENGAN MATRIKS ALGINAT-GELATIN (1,25 : 1,25)% (Dibuat dengan Metode Gelasi Ionik Teknik Aerosolisasi). Skripsi thesis, Universitas Airlangga.

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Abstract

The study was to investigated the characteristics of nisin microsphere which was made from the alginate-gelatine polymer (1.25 : 1.25)%. The parameter of physical characteristics which was carried out includes the morphology, yield, moisture content, particle size, swelling index, and to prove it, it was done by using FTIR and x-ray diffraction (XRD). Nisin microsphere was made with ionotropic gelation method with aerosol technique using calcium chloride (CaCl2) 1.5 M as the crosslinker and the time consumed for the process was 90 minutes. The microsphere formed was dispersed in maltodextrin solution of 5% as lyoprotectant and dried using freeze-drying method. The result of nisin microsphere evaluation showed that the microsphere has spherical shape and smooth surface. The yields were F1= 31.61 ± 2.99% and F2 = 67.85 ± 14.09%. The moisture contents were F1= 9.24 ± 0.34% and F2= 12.37 ± 1.02%. The particle measurements of F1= 3.35±0.15μm and F2 = 5.01±0.10 μm. Swelling index of F1= 386.00±34.18% and F2= 382.33±37.36%. Based on the statistical analysis using T-Test method with 95 % (α = 0.05) degree of trust indicated an impact of gelatine addition to the formula which eventually gave effect to yield, moisture content, particle size and swelling index. Keywords: Nisin, microsphere, alginate, gelatin

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