RINA PUJIASTUTI, 061314153010 (2016) SUPLEMENTASI INSULIN TRANSFERRIN SELENIUM PADA MATURASI IN VITRO CUMULUS OOCYTE COMPLEX TERHADAP EKSPRESI SITOCROM-C dan EKSPRESI CASPASE 3. Thesis thesis, Universitas Airlangga.

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Abstract

This study aimed to detect the role of Insulin Transferrin Selenium against to the increasing oocytes maturation in molecular process with the reduce of citocrom c and caspase 3 expression in oocytes maturation with immunocytochemical by avidin biotin complex method. Oocytes were maturated treatment by maturation media (TCM-199 that were added FSH 0,01 μg/ml, LH 0,01 μg/ml, BSA 3%) were added by ITS with diffrentiated doses. P0: ITS 0 μg/ml, P1: ITS 10 μg/ml, P2: ITS 15 μg/ml, P3: 20 μg/ml. Oocytes maturation were carried on 38.5 0C in incubator CO2 5% for 24 hours. The result supplementation ITS P0 (0 μg/ml) was significantly different (p<0,05) with P1(10 μg/ml), P2 (15 μg/ml) and P3(20 μg/ml) in reduced the expression of cythocorme c and caspase 3 in Cumulus Oocyte Complex (COC). Both expression of cythocrome c was significantly differen (p<0,05), at P2 (15 μg/ml) and P3(20 μg/ml). The conclusion of ITS supplementation experimental on maturation medium is be able to decreased expression of cytochrome c and caspase 3 in oocytes maturation.

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