DESAK KETUT ERNAWATI, 059611864
(2000)
PENGARUH KONSENTRASIION Cd2 +
TERHADAP PERTUMBUHAN DAN KANDUNGAN
FITOSTEROID DARI KULTUR KALUS
COSTVS SPECIOSUS (KOENl F8.
Skripsi thesis, Universitas Airlangga.
Abstract
To more understand the faclors which influeoce phytosteroid biosynthesis, in this work the influeoce of cadmium ions on the growth and phytoslcroid contenl of callus cullures of Costus speaiosus was studied. The in vitro cullurc. were cultivated on modified Murashige Skoog containing 2 mgIL Kinetin and 0,5 mgIL 2,4 D, with the addition of 0,0.5, 1,25, 10, and 20 ppm cadmium ions.
The Growth Index of these callus cultures seemed 10 decrease with the
mcreasement of cadmium ions concentration, following cubic regression
equation. The maximum value of the growth indeks occurred in control cultures with no cadmium addition while the minimum value was occurred in the cultures with addition of20 ppm cadmium ions.
Microscopic investigation on callus biomass by adding diluted HCl and No,S showed that the treated con. contained cadmium ions. Qualitatively analysis of diosgenin by TLC showed there was no diosgenin found in hydrolysate fraction of control and treated culture, whereas sterols were detected Further investigation using Gas Chromatography showed moos slerol. conleol among control cullures and treated cultures.
Densitometric method .... used 10 analyze fu:e sterols and bounded sterols. The highest level of fu:e sterols and bounded sterols were in the treated cultures with 0,5 ppm cadmium iOllll. The lowest level of free sterols was in control cuI"".., Whereas bounded sterols was in treated culture. with 20 ppm ofcadmium ions.
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