Single Cell Sexing Using a Part of Metal Razor Blade of Bovine Embryos Through Male Specific Repetitive DNA

Bambang Poernomo S. (2016) Single Cell Sexing Using a Part of Metal Razor Blade of Bovine Embryos Through Male Specific Repetitive DNA. Global veterinaria, 16 (1). pp. 67-70. ISSN 1992-6197

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Abstract

The development of quick and sensitive methods for sexing of bovine embryos in early stage would be considerable in economic advantages. Polymerase Chain Reaction (PCR) were used for amplification of DNA sequenced derived from the male sex chromosome. The sexed method was allows to removed single cell biopsy using a part of metal razor blade from bovine embryos in less than two minutes without compromise developmental potential. The embryo biopsy can be used for male sex determination. Definite signals following PCR amplification were obtained in all cases indicated that single blastomere cell from preimplantation of bovine embryos was sufficient for male sex determination. Male sex determination of the embryos was developed further in both in vitro and in vivo successfully.

Item Type: Article
Uncontrolled Keywords: Single Cell, Bovine Biopsy, Sex Determinant
Subjects: S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine
Divisions: 06. Fakultas Kedokteran Hewan
Peer Review
Creators:
CreatorsNIM/NIDN
Bambang Poernomo S.UNSPECIFIED
Depositing User: Ika Rudianto
Date Deposited: 20 Jul 2017 18:39
Last Modified: 20 Jul 2017 18:39
URI: http://repository.unair.ac.id/id/eprint/59276
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