Meilia, Wulandari and Masyithoh Budi, Dewi and Ditya Ayu Intan, Setiowati and Duhita Pramesti, Hayuningtyas and Kartika Afrida, Fauzia and Purwanto, Bambang (2014) Curcumin Menghambat Karbonilasi Protein Lensa pada Model Tikus Diabetes yang Diinduksi Streptozotocin. Majalah Ilmu Faal Indonesia (The Indonesian Journal of Physiology), 11 (1). pp. 19-24. ISSN 0215-1995

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Abstract

Aim : lens protein carbonylation is one of mechanism th at involved in complication cataract of diabetes. T he aim of this study was proving the effect of curcumin antio xidant to inhibit lens protein carbonylation in dia betic mouse models. Material and Method: curcumin was solved in maize (corn) oil with concen tration 0.4 mL/100 mg curcumin. Diabetic mouse models were induced by intraperitone al Streptozotocin injection with dose 150 mg/kg of body weight. Curcumin was given once a day as long as two days t o the mouse that had glucose level more than 200 mg /dL. The effect of curcumin was compared to control group, a qua treated group, metformin group and curcumin+met formin group. Lens of diabetic mouse models were pounded t o get homogenate that will be examined the carbonyl protein level. Absorbance was read using spectrophotometer with wave length 375 nm. Result and Discussion: Carbonyl protein levels (nm/ml) were like below: Control gro up (33.16± 19.83), Diabetes+Curcumin group (32.07± 9. 52), Diabetes+Metformin (98.61± 17.72), Diabetes+Curcumin +Metformin group (57.27± 12.19), Diabetes+aquades gr oup (311.83± 73.69). Carbonyl protein level of diabetic mouse lens that given curcumin was not different wi th control group and curcumin+metformin group. Carbonyl protein leve l of diabetic mouse lens that given curcumin was si gnificantly different with aqua treated group and metformin gro up. Conclusion: curcumin inhibits carbonylation of lens protein in diabetic mouse models that induced by streptozotoci n. Suggestion: this experiment must be improved more in order to give option preventive therapy of cataract compl ication of diabetes.

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