Mastutik, Gondo and Juniastuti and Ali Rohman and Amin, Mochammad and Budi Setiawan, Poernomo (2015) Ragaman Genetik Gen Polimerase Virus Hepatitis B Pada Pasien Hepatitis B Kronik dengan Pengobatan Telbivudine. Indonesian Journal of Clinical Pathology and Medical Laboratory, 21 (2). pp. 138-144. ISSN 0854-4263

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Abstract

Infection caused by hepatitis B virus (HBV) is still a major global health problem and can cause liver cirrhosis and hepatocellular carcinoma as well. Telbivudine is one among the drugs used to treat the disease routinely. However, using this drug in a long term therapy might cause mutations in HBV polymerase gene that decreases the effectiveness of the therapy. Here with the researchers report the genetic variations of the gene isolated from telbivudine-which is used treated chronic hepatitis B patients in Surabaya, Indonesia. The blood sera were collected at Dr. Soetomo hospital from 10 telbivudine-treated and 10 untreated chronic hepatitis B patients. The DNA viral was isolated and purified from each serum. Sequence polymerase gene at nucleotides 455 to 796 was amplified by PCR, and then analyzed bio informatically to determine their mutation profile. This study revealed a point mutation in HBV25 sample at nucleotide A1525G that gives rise to I509V modification. Such mutation is also observed in a sequence that is available in Gen Bank with an accession number AY641562. Additionally, the researchers found point mutations A1554G, T1593C, and C1629T in HBV25 sample and a point mutation A1554G in HBV20 sample. However, these mutations are silent. To conclude, the mutation in HBV polymerase gene among telbivudinetreated chronic hepatitis B patients in Surabaya is known as A1525G.

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