Identifikasi Gen KRAS pada berbagai Grade Adenocarcinoma colorectal

Rahniayu, Alphania and Mastutik, Gondo (2014) Identifikasi Gen KRAS pada berbagai Grade Adenocarcinoma colorectal. Project Report. Universitas Airlangga, Fakultas Kedokteran Unair.

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Abstract

Colorectal cancer is one of the most common cancers in the world. Important molecular target for treatment of colorectal cancer is the Epidermal Growth Factor Receptor (EGFR) that treatment with anti-EGFR monoclonal antibody (cetuximab and panitumumab) as a ligand that binds to the EGFR. Cetuximab and panitumumab bind to EGFR and phosphorylated EGFR prevent and block ligand activation-induced EGFR tyrosine kinase, thereby preventing activation of signaling downstream of PI3K and RAS / MEK / ERK pathway. This results in barriers to cellular proliferation and induce apoptosis. This study was an observational study with cross sectional analytic aimed to obtain data identifying the KRAS gene in a variety of dosage colorectal adenocarcinoma grade paraffin blocks Hospital Dr Soetomo. Samples were collected at the Department / SMF / Installation Pathology Faculty of Medicine, University of Airlangga - Hospital Dr Soetomo began January 1, 2013 until December 31, 2013. Profile colorectal malignancy in Dr. Soetomo hospital years 2009-2013 are benign tumors of epithelial cells 65 cases and 48 cases of non epithelium. The number of cases of malignant tumors of epithelial cells 883 cases and 45 cases. Most malignant tumors of the epithelial cells are well differentiated adenocarcinoma 473 cases, poor differentiated adenocarcinoma 137 cases, 79 cases mucinus adenocarcinoma, signet ring adenocarcinoma 43 cases, and moderately differentiated adenocarcinoma 42 cases. Rare cases are squamous cell carcinoma and adenosquamous carcinoma in 3 cases 2 cases. Identifikas KRAS gene in this study can be conducted with pre PCR conditions 5 min denaturation 940C, 940C 1 minute denaturation, annealing 630C 1 minute, 30 seconds extention 720C, for 40 cycles and a final extension 720C 10 menit.Saran proposed in this research is to KRAS gene mutation detection by PCR RFLP teknk because it is easy and relatively inexpensive compared to other techniques.

Item Type: Monograph (Project Report)
Uncontrolled Keywords: adenocarcinoma colorectal, PCR, KRAS
Subjects: R Medicine > R Medicine (General) > R735-854 Medical education. Medical schools. Research
Divisions: 01. Fakultas Kedokteran > Patologi Anatomi
Creators:
CreatorsNIM/NIDN
Rahniayu, AlphaniaUNSPECIFIED
Mastutik, Gondogondomastutik@gmail.com
Depositing User: Bayu Yulianti
Date Deposited: 23 Aug 2017 19:23
Last Modified: 23 Aug 2017 19:23
URI: http://repository.unair.ac.id/id/eprint/60830
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