NI LUH AYU MEGASARI, NIM011514253014 (2017) DISTRIBUSI SUBTIPE HIV-1 PADA PASIEN HIV-POSITIF TREATMENT NAÏVE DAN TREATED DI KABUPATEN BULELENG, BALI. Thesis thesis, Universitas Airlangga.

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Abstract

Human Immunodeficiency Virus (HIV) infection might cause Acquired Immunodeficiency Syndrome (AIDS). Bali Province ranked fifth among provinces with highest HIV cumulative case in Indonesia. Buleleng Regency had the second largest HIV cases in Bali. One of the progression markers related to HIV-1 subtypes is viral load. Antigen p24 concentration had a positive correlation with viral load. Some study suggested the use of antigen p24 concentration measurement to substitute viral load testing in resource limited setting. The purpose of this study was to identify HIV-1 subtypes‟ distribution among treatment naïve and treated patients in Buleleng Regency, Bali. This study also aimed to identify the correlation between HIV-1 subtypes with the concentration of antigen p24. Twenty four plasma samples obtained from HIV-positive treatment naïve and treated patient were subjected to antigen p24 quantification using quantitative ELISA. RNA isolated from the plasma samples and subjected to genotyping. Envelope gene were successfully amplified and sequenced. HIV-1 subtyping revealed that CRF01_AE (75%), subtype B (16,67%), CRF02_AG (4,17%), and subtype A1 (4,17%) circulate in the region. Nineteen patients (70,1%) known to have undetectable antigen p24 concentration, while 5 patients (19,9%) have concentration between >0 to 7,8 pg/ml. No significant correlation found between HIV-1 subtypes and concentration of antigen p24 (p=0.776), even after adjusted by ART status (p=0.386 on treatment naïve patient, p=0.378 on patient treated less than 6 months). This study revealed that CRF01_AE was the predominant HIV-1 subtype circulating in Buleleng Regency, Bali. There was no correlation found between HIV-1 subtypes and concentration of antigen p24

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