Pain Relief with Wet Cupping Theraphy in Rats is Mediated by Heat Shock Protein 70 and β-Endorphin

Subadi, Imam and Nugraha, Boya and Laswati P, Hening and Josomuljono, Harjanto (2017) Pain Relief with Wet Cupping Theraphy in Rats is Mediated by Heat Shock Protein 70 and β-Endorphin. Iranial Journal of Medical Sciences, 42 (4). pp. 384-391. ISSN 0253-0717/1735-3688

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Official URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC55230...

Abstract

Background: Wet cupping therapy is a complementary therapy in pain management. The mechanism of this therapy, however, needs further elucidation. Cells injured by wet cupping therapy seem to stimulate the expression of heat shock protein 70 (HSP70). Its benefit in pain reduction could be mediated by the expression of ß-endorphin. This study aimed at determining the correlation between HSP70 and ß-endorphin after wet cupping therapy. Methods: Sixteen male Wistar rats were divided into control (CG; n=8) and treatment (TG; n=8) groups. The rats in both groups were injected with complete Freund’s adjuvant (CFA) at the footpad. In the TG, wet cupping therapy was done at the left and right paralumbar regions 48 hours after the CFA injection. Twenty-four hours after therapy, the hot plate test was done to assess pain threshold. Thereafter, immunohistochemistry from the skin subjected to wet cupping therapy was conducted for HSP70 and ß-endorphin. Results: The expression of HSP70 was significantly higher in the keratinocytes of the TG (20.25±3.53; P<0.001) than in the keratinocytes of the CG (10.50±2.44; P<0.001). The expression of ß-endorphin was significantly higher in the keratinocytes of the TG (22.37±3.52; P<0.001) than in the keratinocytes of the CG (5.12±1.72; P<0.001). The results also revealed a high correlation between HSP70 and ß-endorphin (β=0.864; P<0.001). Pain threshold after wet cupping therapy was significantly higher in the TG (22.81±6.34 s; P=0.003) than in the CG (11.78±3.56 s). Conclusions: The benefit of wet cupping therapy in terms of pain reduction in rats could be mediated by the expression of HSP70 and ß-endorphin.

Item Type: Article
Uncontrolled Keywords: Pain, Rat, HSP70 heat-shock proteins, β-endorphin, Complementary therapies
Subjects: R Medicine > R Medicine (General) > R735-854 Medical education. Medical schools. Research
Divisions: 01. Fakultas Kedokteran > Ilmu Fisik & Rehab
Creators:
CreatorsNIM/NIDN
Subadi, ImamUNSPECIFIED
Nugraha, BoyaUNSPECIFIED
Laswati P, Heninglputra04@yahoo.com
Josomuljono, HarjantoUNSPECIFIED
Depositing User: Bayu Yulianti
Date Deposited: 11 Sep 2017 23:36
Last Modified: 11 Sep 2017 23:36
URI: http://repository.unair.ac.id/id/eprint/61382
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