REAKSI SILANG ANTIGEN Eurytrema pancreaticum DENGAN ANTIBODI TERHADAP Fasciola gigantica MENGGUNAKAN METODE WESTERN BLOT

DEBBY ADELIA MARISTA, 061311133066 (2017) REAKSI SILANG ANTIGEN Eurytrema pancreaticum DENGAN ANTIBODI TERHADAP Fasciola gigantica MENGGUNAKAN METODE WESTERN BLOT. Skripsi thesis, Universitas Airlangga.

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Abstract

This research is to conduct to examine the cross reaction between Eurytrema pancreaticum antigens and antibodies on Fasciola gigantica using Western blot Technique. Adult Fasciola gigantica worms collected from liver of cattle and Eurytrema pancreaticum collected from pancreas of cattle. Homogenate created from adult Fasciola gigantica and Eurytrema pancreaticum that crushed using mortar and stamfer and added with Phospate Buffer Saline (PBS). Characterization of protein by SDS-PAGE technique with Comasie Brilliant Blue staining. Cross reaction of Eurytrema pancreaticum antigens and anti-Fasciola gigantica using Western blot Technique with western blue staining and IgG anti-mouse and obtained 16 protein bands are 151, 87, 76, 63, 57, 56, 52, 40, 39, 33, 29, 24, 19, 15, 13 and 10 kDa.

Item Type: Thesis (Skripsi)
Additional Information: KKC KK KH.319/17 Mar r
Uncontrolled Keywords: Cross reaction, Eurytrema pancreaticum, Fasciola gigantica, Western blot
Subjects: S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine > SF811-909 Veterinary medicine of special organs, regions, and systems
Divisions: 06. Fakultas Kedokteran Hewan
Creators:
CreatorsNIM/NIDN
DEBBY ADELIA MARISTA, 061311133066UNSPECIFIED
Contributors:
ContributionNameNIDN/NIDK/NUP
ContributorNusdianto Triakoso, Dr., drh., MP.UNSPECIFIED
ContributorKusnoto, Dr., drh., MSi.UNSPECIFIED
Depositing User: Mr Binkol1 1
Date Deposited: 27 Dec 2017 13:21
Last Modified: 27 Dec 2017 13:21
URI: http://repository.unair.ac.id/id/eprint/62181
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