Gita Nirmala (2015) Pengaruh Pemberian Ekstrak Kelopak Bunga Rosella Merah (Hibiscus sabdariffa Linn) Terhadap Kadar Malondialdehid (MDA) Plasma Darah Pada Mencit (Mus Musculus) Jantan yang Terpapar Pb asetat. Skripsi thesis, Universitas Airlangga.

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Abstract

Lead acetate is a heavy metal. It absorption through respiratory tract, gastrointestinal tract, skin contact and in the body can be accumulated in tissue. There are many studies that have shown that lead causes oxidative stress by inducing the generation of reactive oxygen species and reducing the anti-oxidant defense system. Excessive free radicals will cause oxidative stress that triggers the process of lipid peroxidation and oxidative damage. Rosella contains vitamin C, vitamin E, antosianin and flavonoids, is a natural antioxidant which can reduce the negative impact of oxidants including free radicals. This research aims to determine the effect of rosella petal flower extract against MDA in mice fed with waste lead acetate. Decrease in oxidative stress can be determined by measuring MDA which is the end product of lipid peroxidation. Twenty male mice, 20 g body weight were divided into five groups. Group I served as negative control, group II served as positive control, group III to V were given lead rosella petal flower extract doses 200, 400, 800 mg/kg BW and lead acetate at dose 20 mg/kg BW. After 28 days, plasma malondialdehyde levels were measured. Statistical analyses was done by one way ANOVA followed by Tamhane Test. The plasma level P1, P2 and P3 group showed insignificant reduction compared to the positive control group. There were insignificant differences in MDA levels decreased in all five groups after the treatment rosella petal flower extract administration orally for 28 days (p<0.05). It can be concluded, that the rosella petal flower extract administration dose of 200 mg/kg BW, 400 mg/kg BW and 800 mg/kg BW can not reduce levels of MDA in mice fed with waste lead acetate. The result of this study is expected to be used as a basis for further research to determine the maximum dose of rosella petal flower extract in experimental animal.

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