MEKANISME KERJA EKSTRAK ETANOL BATANG TORBANGUN (Coleus amboinicus) TERHADAP MODEL NEFROPATI AKIBAT INDUKSI ASAM URAT PADA TIKUS PUTIH (Rattus norvegicus)

RONDIUS SOLFAINE, NIM011317017305 (2017) MEKANISME KERJA EKSTRAK ETANOL BATANG TORBANGUN (Coleus amboinicus) TERHADAP MODEL NEFROPATI AKIBAT INDUKSI ASAM URAT PADA TIKUS PUTIH (Rattus norvegicus). Disertasi thesis, Universitas Airlangga.

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Abstract

Introduction. Torbangun (Coleus amboinicus, CA) extracts are known to have anti-oxidant activity, anti-platelet aggregations, anti-bacterial, anticancer and antiinflammation. Aim. The purposed to study the mechanism of Coleus amboinicus (CA) stem extracts in Wistar rats with uric acid-oxonic acid induced nephropathy, by analyzing the levels of Glutathione Peroxidase (GPx) activity, Interleukin-1β (IL- 1β), Transforming growth factor-β1 (TGF-β1) expression and macrophage CD 68 (ED-1). Method. Fifty-six male Wistar rats (Rattus norvegicus), 3 months with 150-200 g body weight, were allocated into four groups (n=14). The control group received placebo, treatment group were administered orally with uric acid 1,5% and oxonic acid 2% (P1) and received 500 mg/kg BW of the CA stem extracts (P2) and received 5 mg/kg BW quercetin (P3) by oral, respectively for 35 days. Blood serums collected for analysis of cystatin C (Cys-C), creatinine and BUN concentrations. All groups were sacrificed to collect kidney organ for measuring of GPx activity, IL-1β expression, TGF-β1 expression was conducted by Avidin- Horseradish Peroxidase (HRP) Sandwich-ELISA. Kidney organ was collected to histopathological analyzed by Hematoxylin-eosin and Periodic Acid Schiff staining. Immunohistochemistry is conducted for identification of CD 68 expression. The levels of GPx, IL-1β, TGF-β1, CD 68 and Cys-C concentrations were analyzed by statistic independent test. Results. Coleus amboinicus (CA) stem extract by Thin Layer Chromatography has a relative fraction of flavonoids, terpenes, saponins, polyphenols and alkaloids. By in silico analyzed, quercetin as a marker compound of CA stem extract has stronger bind to the TGF-β1receptor (PDB code: 4X0M) than its of 3WA_601 ligand. Induction with uric acid- oxonic acid for 35 days has proven to causes nephropathy with indicated of elevated BUN, creatinine and cystatin-C levels and necrotic lesions of tubular membrane and glomerular kidney, in P1, P2, and P3 groups. The research results showed that CA stem extract at a dose of 500 mg/kg BW decreased the activity of GPx, IL-1β expressions and CD 68 not significantly (p> 0.05) and increased of TGF-β1 expression significantly (p≤0.05) in P2 and P3 groups than the P1 group. This study was concluded that torbangun stem extract is proven to inhibit on uric acid-induced nephropathy, however, a mechanism of that inhibitory activity could not explain clearly in this research.

Item Type: Thesis (Disertasi)
Additional Information: KKA KK Dis.K.30/17 Sol m
Uncontrolled Keywords: Coleus amboinicus, Wistar rat, nephropathy, in silico, mechanism of action
Subjects: R Medicine > R Medicine (General) > R735-854 Medical education. Medical schools. Research
Divisions: 01. Fakultas Kedokteran
Creators:
CreatorsNIM/NIDN
RONDIUS SOLFAINE, NIM011317017305NIM011317017305
Contributors:
ContributionNameNIDN/NIDK/NUP
ContributorEndang Joewarini, Prof. Dr., dr.,Sp.PA (K)UNSPECIFIED
ContributorSiswandono, Prof. Dr., Apt., MSUNSPECIFIED
Depositing User: Mr Binkol1 1
Date Deposited: 27 Dec 2017 18:15
Last Modified: 18 Jan 2018 21:10
URI: http://repository.unair.ac.id/id/eprint/63535
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