SAFIRA ADILLA, 051311133086 (2017) PENGARUH KONSENTRASI PENYAMBUNG SILANG CaCl2 TERHADAP KARAKTERISTIK MIKROSFER ERITROPOETIN-ALGINAT. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The aim of this research was to investigate the effect of CaCl2 concentration on the characteristics (particle size, morphology, swelling index, and yield) of erythropoietin-alginate microspheres prepared by ionotropic gelation method with aerosolization technique using sodium alginate as polymer and CaCl2 as crosslinker. Erythropoietin-alginate microspheres formed were dried using freeze drying method with maltodextrin as lyoprotectant. The concentrations of alginate used were 2%, and CaCl2 concentrations were 0.5 M, 0.75 M and 1 M. Results showed smooth and spherical microspheres for all formula with average particle size were 3.23±0.05 μm (F1); 2.99±0.07 μm (F2); and 2.86±0.03 μm (F3). Mass swelling index at 24 hours were 1.25±0.10 (F1), 1.18±0.11 (F2), and 1.11±0.10 (F3); at 30 hours were 2.00±1.25 (F1), 1.85±0.14 (F2), and 1.72±0.15 (F3) while particle size swelling index at 24 hours were 1.15±0.10 (F1), 1.11±0.10 (F2), and 0.97±0.10 (F3); at 30 hours were 1.81±0.09 (F1), 1.73±0.15 (F2), and 1.54±0.14 (F3). Respectively yield percentage were 77.76±6.49 % (F1), 80.01±3.53 % (F2), and 82.97±4.22 % (F3). By using One Way ANOVA, it was found that there were significantly differences between three formulas. The particle size of formulas decreased by increasing concentration of CaCl2, whereas no significant difference on swelling index and yield from microspheres with increasing CaCl2 concentration simultaneously.

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