ANALISIS MOLEKULER VIRUS HEPATITIS A DARI SAMPEL KLINIK PASIEN HEPATITIS AKUT DI JAWA TIMUR PADA TAHUN 2015-2016

Fadhilah Mega Indriati, 011311133230 (2016) ANALISIS MOLEKULER VIRUS HEPATITIS A DARI SAMPEL KLINIK PASIEN HEPATITIS AKUT DI JAWA TIMUR PADA TAHUN 2015-2016. Skripsi thesis, Universitas Airlangga.

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Abstract

INTRODUCTION: Acute hepatitis is an infectious disease mainly by hepatitis A virus which has 7 genotypes. Nevertheless there is no study revealing the genotype distribution of hepatitis A virus yet in Indonesia. It is reported that outbreak has happened in 2015 in Bogor and Bondowoso. Therefore it is imperative to investigate the virus distribution to suppress infection cases of acute hepatitis patients from Bondowoso in which outbreak have taken place at late November 2015 till the beginning of 2016. METHODS: Sera sample was carried out by the ELISA. The positive sera of anti-HAV were subjected to RT-PCR to detect HAV RNA and then direct sequencing. The HAV genotype was examined sequencing scanner in VP1/P2A regio and VP3/VP1 regio. Then, it analyze by phylogenetic analysis. RESULTS: Result anti-HAV reveals 100% of samples (16 samples) are infected by hepatitis A virus. Despite that through genotype analysis only 50% (8 from 16 samples) have RNA detectable and indicate genotype 1A infection. DISCUSSION: This study shows that HAV outbreak in Bondowoso is caused mainly by genotype 1A. it has similar subgenotype with other HAV infection in Indonesia and Japan’s case wich is infected during his time in Surabaya. In spite of that there is no research yet focusing on characterization of the virus and its distribution pattern in Indonesia. Thus it is urgent to find hepatitis A vaccine to thwart this virus infection, prevent the large case and as a parameter for the rule of government. CONCLUSION: Hepatitis A virus genotype 1A is the dominant strain responsible for infection cases in Indonesia. The epidemiological data of each case should be reported and researched.

Item Type: Thesis (Skripsi)
Additional Information: KKA KK FK.PD.141/17 Ind a
Uncontrolled Keywords: HAV, PCR, ELISA, outbreak, East Java, Indonesia
Subjects: R Medicine > RA Public aspects of medicine > RA1-1270 Public aspects of medicine > RA421-790.95 Public health. Hygiene. Preventive medicine > RA648.5-767 Epidemics. Epidemiology. Quarantine. Disinfection
Divisions: 01. Fakultas Kedokteran
Creators:
CreatorsEmail
Fadhilah Mega Indriati, 011311133230UNSPECIFIED
Contributors:
ContributionNameEmail
ContributorMaria Lucia Inge Lusida, Prof., dr., MKes., Ph.D., Sp.MK(K)UNSPECIFIED
ContributorHarianto Notopuro, Prof. Dr., dr., MSUNSPECIFIED
Depositing User: Mr Binkol1 1
Date Deposited: 30 Dec 2017 15:43
Last Modified: 30 Dec 2017 15:43
URI: http://repository.unair.ac.id/id/eprint/66236
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