Haris Swastikoputra, NIM011311133070 (2017) PERBEDAAN KONTAMINASI Salmonella sp. DAN MIKROBA TIDAK SPESIFIK PADA DAGING SAPI DI PASAR PAGI DAN PASAR SIANG KECAMATAN TAMBAKSARI SURABAYA TIMUR TAHUN 2016. Skripsi thesis, Universitas Airlangga.

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Abstract

The traditional market is synonymous with a seedy, dirty and disorganized. Especially in the market that sells meat, lots of flies fluttering to the floor muddy and dirty. Among the traditional market are morning market and afternoon market. One of the merchandise sold in the market is the beef. Damage causes a decrease in the quality of fresh meat, mainly caused by microorganisms. One of bacterial microorganisms that can contaminate beef is Salmonella sp. Salmonella sp. can causes Salmonellosis. One of the manifestations in class typhoidal species known to many people as typhoid fever. In 2007, the CDC reported the prevalence of typhoid fever cases in Indonesia is around 358-810 per 100,000 people, with 64% between the ages of 3 to 19 years. For three consecutive years since 2007, Salmonella sp. encountered as a cause of food poisoning in Indonesia and likelihood of occurrence ranged from 12,5% to 25% of microbial contamination. The test results indicate that the presence of Salmonella on beef sold in markets in West Java province is 54,2%. Therefore, research related to contamination with Salmonella sp. within beef needs to be done on specific areas such as in the traditional markets. Type of research that used in this study is an analytic observational with cross sectional approach. This study is an analytical. Sampling method used in this study is cluster sampling. The research subjects were obtained as many as 32 samples. This research is collecting primary data via vendors information, isolation and identification test of Salmonella sp., and Total Plate Count (TPC) test. The variables studied were the duration of the merchants selling, the duration of storage of the meat, merchants hygiene, sanitation where the sale of meat, sanitary appliance merchants belong to, suspension of meat, types of butcher, types of markets, contamination of Salmonella sp., and non-specific microbial contamination. From the total 32 samples were tested, it was found 7 positive samples contaminated with Salmonella sp. (21,9%). Contamination of Salmonella sp. more abundant in beef from the afternoon market (37,5%) than beef from the morning market (6,3%). Fisher’s Exact test results showed no significant differences for p values > 0,05 (p = 0,83). Of the total 32 samples tested with TPC method, it was found the average number of non-specific microbial colonies of 0,36 x 105 cfu/g on the beef from the morning market and at 1,7 x 105 cfu/g on the beef from the afternoon market. Independent Samples T test results showed that there were significant differences for p <0,05 (p = 0,018). This showed that there are no significant differences in contamination of Salmonella sp. and there were significant differences in non-specific microbial contamination on beef beetween the morning market and afternoon market Tambaksari District, East Surabaya in 2016. The next study expected to involve a larger number of samples, perform detection of contamination since the beginning of the abattoir and use criteria more specific variables, also exclude other confounding factors such as temperature, dryness level of meat, so that samples can represent the condition of the study population more accurately.

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