FENITA SHOVIANTARI, 051424153003 (2017) EFEKTIVITAS, IRITABILITAS, DAN STABILITAS FISIK COENZYM Q10 DALAM SISTEM PENGHANTARAN NANOEMULSI DAN NANOSTRUCTURED LIPID CARRIERS SEBAGAI KOSMETIKA ANTIAGING. Thesis thesis, Universitas Airlangga.

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Abstract

Coenzyme Q10 is an antioxidant that has activity as antiaging agent with the mechanism of increasing production of basal membranes, fibroblast proliferation, and protect skin from UV damage. Coenzyme Q10 has some problem when formulated in topical dossage forms, such as low water solubility (0.193 g/ml in water), a high molecular weight (863.36 g/mol), and high lipophilicity (log P> 10) so it makes coenzyme Q10 retained in stratum corneum and causing low skin penetration. In this research, coenzyme Q10 was prepared in two different delivery system that is nanoemulsion as nanosystem based emulsion and nanostructured lipid carriers (NLC) as nanosystem based lipid particle and focused on in vivo effectivity and irritability, also physical stability in each system. Nanoemulsion and NLC characterization was conducted, that is, viscosity using cone and plate viscometer, pH using pHmeter, particle size and polydispersity index using Delsa Nano Particle Size Analyzer, droplets or particles morphology using Transmission Electrone Microscopy (TEM), and entrapment efficiency of NLC coenzym Q10 using sentrifuge method with spectrophotometer UV in 271 nm wavelength. Effectivity test was determined by depth of penetration after 2, 4, and 6 hours sample application in back mice skin using fluorescent microscopy with Rhodamine B as fluorescent label, and antiaging activity after 14 days sample application in back mice skin that was measured by scoring of collagen density and number of fibroblast in histopathological preparation in Masson Trichrome staining that observed by light microscopy. The irritability test after 24 hours sample application in back mice skin was measured by scoring histopathogy in Hematoxilin Eosin staining that observed by light microscopy. And, physical stability of each delivery system determined based on minimum of particle size, polydispersity index, and pH variation during 45 days storage in room temperature. The result showed that there were no significant differences between coenzyme Q10 in nanoemulsion and NLC in effectivity (in antiaging activity) and irritability score, but coenzyme Q10 in NLC had shown more stable in 45 days storage than in nanoemulsion.

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