Zahreni Hamzah, 091170124 (2017) PENINGKATAN JUMLAH SEL PUNCA MESENKIMAL LIGAMEN PERIODONTAL PENGHASIL HSP-60 dan TGF-β BERPERAN PADA MOBILITAS GIGI TIKUS SPRAGUE-DAWLEY YANG MENGALAMI DISTRES KERJA KRONIS. Disertasi thesis, Universitas Airlangga.

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Abstract

Background. Tooth mobility is often found in workers with chronic work distress. However, up to the moment, tooth mobility in workers with chronic work distress, has not been disclosed. If it is not taken care properly, tooth mobility due to chronic work distress will lead to premature tooth loss. Based on the workloads, workers can be classified those who are at upper middle class who are likely to experience psychological distress, and those at lower middle class who are more likely to experience psychological and physical work distress. Objective. This study was to explain tooth mobility that are often found in workers with chronic work distress. This study used male Rattus norvegicus of Sprague-Dawley strain aged 3 months as worker model. Methods. This was an experimental laboratory study using Randomized Separate pretest-posttest control group design. The rats were grouped into four groups: Pretest (P), post-test, consisting of control (K), psychological distress (SP), and psychological-physical distress group (SPF), each of which consisted of eight rats. Stressor was provided with electric foot shock generator (48 V, 0.5 Hz; 2-5 mA) for 30 minutes for 7 days in a communication box. Examination of intra-cell signaling in the periodontal ligament stem cells was performed with IHK. Data were processed with multivariate analysis of variance and path analysis. Results. Path analysis showed that the electric shock resulted in increased perception of stress to become distress through the stimulation of the HPA axis, which induced distress response in the form of increased corticosterone. Increased levels of corticosterone in psychological distress increased the number of Hsp-60 and TGF-β expressing PDLSC, which directly resulted in increased the apoptosis of fibroblasts, osteoblasts and decreased collagen density; while in psychological-physical distress treatment direct increase occurred in TGF-β expression that resulted in a decrease in cementoblast and osteoblasts, which decreased the thickness and density of collagen, thereby triggering the onset of tooth mobility. Conclusion. Psychological and psychological-physical work distress may induce tooth mobility through increasing expression of Hsp-60 and TGF-β producing by PDLSC, which resulted in a decrease in the number of fibroblasts, osteoblasts, cementoblas, as well as a decrease in thickness and density of type-1 collagen, thereby the binding of tooth root into the socket bond reduced.

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