Sony Wibisono Mudjanarko, 091070125 (2017) PENGARUH ULTRA VIOLET C TERHADAP PENINGKATAN HEAT SHOCK PROTEIN 70 (HSP70) DAN SIX TRANSMEMBRAN PROTEIN PROSTAT2 (STAMP2) PADA SEL PUNCA MESENKIMAL ADIPOSA MANUSIA OBESITAS. Thesis thesis, Universitas Airlangga.

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Abstract

Background: Obesity is known increased inflammatory cytokines, STAMP2 a modulator to an increase in TNFα decreased and HSP70 decreased in obesity. Heat stress with ultra violet (UV) might have benefit increased HSP70. Objective: To evaluate effect of UVC in the increase intracellular HSP70 and STAMP2 mesenchyme stem cells (MSCs) adipose obese human. Method: the experimental invitro study of mesenchyme stem cells of obesity at the Institute of Tropical Diseases (LPT) Airlangga University. MSCs cultures were divided into 4 groups for examination HSP70, STAMPβ, TNFα, IL6 and GLUT4. Groups is divided to 0 minutes, 10 minutes, 20 minutes and 30 minutes as exposure of UVC. The significance level was measured p <0.05. Results: UVC increase HSP70, STAMP2, IL6 significantly (p = 0.01, p = 0.01, p = 0.01) and lower TNFα (p = 0.01). Time positively correlated with HSP70, STAMPβ, IL6 significantly (r = 0.85, p = 0.01; r = 0.70, p = 0.01; r = 0.83, p = 0.01). Time negatively correlated with TNFα (r = - 0.93, p = 0.01). Correlation HSP70 with STAMP2 and IL6 significantly (r = 0.49, p = 0.016 ; r = 0.79, p = 0.01). Correlation STAMP2 with IL6 significantly (r = 0.655, p = 0.001). Role of UVC to HSP70, STAMPβ, TNFα and IL6 significantly (β = 0.86, p <0.05; β = 0.49, p <0.05; β = - 0.5β, p <0.05; β = 1.γγ, p <0.05). Conclusions: UVC increases HSP70, STAMP2, and IL6 significantly and lower the TNFα significantly in MSCs obese. HSP70 positive role with STAMPβ significantly, STAMPβ negative role against TNFα significantly.

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