EFEKTIFITAS EKSTRAK PROPOLIS TERHADAP EXTRACELLULAR POLYMERIC SUBSTANCE (EPS) BIOFILM BAKTERI Enterococcus Faecalis

DHEA ADITTYA, drg, 021418036309 (2017) EFEKTIFITAS EKSTRAK PROPOLIS TERHADAP EXTRACELLULAR POLYMERIC SUBSTANCE (EPS) BIOFILM BAKTERI Enterococcus Faecalis. Thesis thesis, Universitas Airlangga.

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Abstract

Backgorund: The resistance of Enterococcus faecalis bacteria in root canals is because it can form a biofilm. Biofilms are communities of microorganisms in a complex and dynamic structure attached to a surface, embedded in Extracellular Polymeric Substance (EPS). Propolis extract contains of flavonoids that can inhibit biofilm growth. Objective: To analyze the effectiveness of propolis extracts against Enterococcus faecalis biofilm EPS in vitro. Materials and Methods: Enterococcus faecalis biofilm grown on media Tryptic Soy Broth (TSB), dextran conjugate alexa fluor 647 reagent was added, then incubated 24 hours. Enterococcus faecalis biofilm were divided into 4 groups: 3 groups treated by soaking propolis extracts 24 hours with each concentration of 02, 0.8% and 1.2%; 1 control group without extract of propolis. Biofilm samples examined using Confocal Laser Scanning Microscope (CLSM). Yield data was then analyzed using One Way ANOVA and Tukey HSD Test (p <0.05). Results: The treatment group showed a decrease in the average volume of biofilm EPS than the control group. Conclusion: propolis extracts with concentration of 0.2% 0.8% and 1.2%, effectively reducing the Enterococcus faecalis biofilm EPS.

Item Type: Thesis (Thesis)
Additional Information: KKA KK PPDGS.KON.16/17 Adi e
Uncontrolled Keywords: Biofilm, Enterococcus faecalis, extracellular polymeric substance, propolis
Subjects: R Medicine > RK Dentistry
Divisions: 02. Fakultas Kedokteran Gigi > Concervative
Creators:
CreatorsNIM/NIDN
DHEA ADITTYA, drg, 021418036309UNSPECIFIED
Contributors:
ContributionNameNIDN/NIDK/NUP
ContributorLatief Mooduto, Prof. Dr., drg., MS., Sp.KG(K)UNSPECIFIED
ContributorAri Subiyanto, drg., M.Kes., Sp.KG(K)UNSPECIFIED
Depositing User: Mr Binkol1 1
Date Deposited: 31 Dec 2017 16:02
Last Modified: 31 Dec 2017 16:02
URI: http://repository.unair.ac.id/id/eprint/66905
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