SINARI ALFAT SUNARKO, 091315053002 (2017) POTENSI FOTOSENSITISIZER FEOFITIN A UNTUK INAKTIFASI STREPTOCOCCUS MUTANS DENGAN LASER DIODA 405 NM (Hasil Pemecahan fraksi klorofil daun Pleomele angustifolia). Thesis thesis, Universitas Airlangga.

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Abstract

This research aimed to know the potency of pheophytin a as a photosensitizer agent to inactivated Streptococcus mutans using 405 nm diode laser. Pheophytin a is known as chlorophyll a derivate that loss magnesium ion at the center of porphyrin ring structure. Porphyrins are commonly employed for aPDT as photosensitisizer. Pheophytin a was obtained from the chlorophyll breakdown of Pleomele angustifolia leaves. Methods of this research were pheophytin a isolation, laser characterization, toxicity test of 20 % pheophytin a solution and photoinactivation of Streptococcus mutans using 405 nm diode laser with doses of 2.5, 5, 7.5, 10, 12.5, 15.0, 17.5 and 20.0 J/cm2. Pheophytin a obtained from Pleomele angustifolia leaves characterized using TLC, UV-Vis spectra and infrared spectra profiles. Streptococcus mutans incubated with pheophytin a without photoinactivation resulted no significant death (untoxic). The final result of this research showed that photoinactivation of Streptococcus mutans incubated with pheophytin a caused the reduction of Streptococcus mutans colonies greater than laser exposure without pheophytin a incubation at the same dose of diode laser. The greatest percentage of Streptococcus mutans reduction occurred at the group of Streptococcus mutans incubated with pheophytin a dan exposured with 20 J/cm2 diode laser. The reduction was 62%.

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