Osteogenic Ability of Combined Hematopoetic Stem Cell, Hydroxyapatite Graft and Platelet Rich Fibrin on Rats (Rattus Novergicus)

Dwi, Rahmawati and Retno Indrawati, Roestamadji and Ernie Maduratna, Setiawatie and Anita, Yuliati and Toufan, Bramantoro (2017) Osteogenic Ability of Combined Hematopoetic Stem Cell, Hydroxyapatite Graft and Platelet Rich Fibrin on Rats (Rattus Novergicus). JKIMSU Vol. 6, No. 4, October-December 2017, 6 (4). pp. 88-95. ISSN 2231-4261

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Abstract

Background: In Indonesia, the incidence rate of tooth extraction as the main form of dental treatment reached as high as 79.6% in 2014. Teeth extraction leads to periodontal tissue defect whose treatment, incorporating the use of graft material Hydroxyapatite (HA) and Platelet Rich Fibrin (PRF) with stem cells, has become increasingly widespread. The stem cell potentially put to therapeutic use is the Hematopoetic Stem Cell (HSC). Aim and Objectives: to examine the effect of hematopoetic stem cell addition to HA graft and PRF post-tooth extraction on the number of osteoblasts and amount of Osteoprotegerin (OPG) in periodontal defect treatment. Material and Methods: This study constituted laboratory-based experimental research with a post test-only control group. Twenty four rats (Rattus novergicus) represented the animal study model in this research. Alveolar bone defect in the animal study model was induced by extracting the first molar of the mandible using sterile needle holder clamps. The sample population was divided into four groups: K0: the untreated control group; K1: socket filled with HA Graft and PRF. K2: socket filled with HSC 105 cells. K3: the socket filled with HAGraft and PRF with HSC 106 cells. Results: The K3 group had both the highest number of osteoblasts when compared with the control group (350.17 ± 33.42; P <0.001) and the strongest OPG expression of all treatment groups (11,36 ± 0,54; P <0.01). Conclusion:Acombination of HA graft, PRF and HSC significantly increases the Osteogenic Ability of Combined Hematopoetic Stem Cell, Hydroxyapatite Graft and Platelet Rich Fibrin on Rats (Rattus Novergicus) Dwi Rahmawati1, Retno Indrawati Roestamadji , Ernie Maduratna Setiawatie3, Anita Yuliati4, Taufan Bramantoro5 1Oral and Dental Science, 2Department of Oral Biology,3Department of Periodontic, 4Department of Dental Material and Technology, 5Department of Dental Public Health, Faculty of Dental Medicine, Universitas Airlangga, Surabaya-Indonesia 2* expression of OPG and the number of osteoblasts rendering it a potential treatment for tissue engineering-based periodontal defect. Keywords: Osteoprotegerin, Osteoblast, Bone regeneration, Bone graft, Tissue Engineering

Item Type: Article
Uncontrolled Keywords: Osteoprotegerin, Osteoblast, Bone regeneration, Bone graft, Tissue Engineering
Subjects: R Medicine
R Medicine > RK Dentistry
Divisions: 02. Fakultas Kedokteran Gigi > Dental Public Health
Creators:
CreatorsEmail
Dwi, RahmawatiUNSPECIFIED
Retno Indrawati, RoestamadjiUNSPECIFIED
Ernie Maduratna, SetiawatieUNSPECIFIED
Anita, YuliatiUNSPECIFIED
Toufan, BramantoroUNSPECIFIED
Depositing User: Rudy Febiyanto
Date Deposited: 06 Feb 2018 21:19
Last Modified: 15 Jul 2018 19:36
URI: http://repository.unair.ac.id/id/eprint/69420
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