LUKITA AFRIDA, 061514253016 (2018) AKTIVITAS ANTIMIKROBA DARI SEKRESI CRUDE PROTEIN Saccharomyces cerevisiae YANG TERDAPAT PADA SUSU KUDA SUMBAWA PENELITIAN EKSPLORATIF LABORATORIK. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The aim of this study was to know any yeast in Sumbawa mare milk, the antibacterial effects against Escherichia coli and Staphylococcus aureus, and the secretory protein profile based on molecular weight by SDS-PAGE. Yeast was isolated by conventional methods in Saboraud Dextrose Agar (Merck), plates containing 0.05 mg/ml of chloramphenicol to inhibit bacterial growth and incubate at 37°C in 48 hours. Identification process include macroscopic, microscopic, biochemically test, and urea hydrolysis test. Antimicrobial activity was tested against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923. Molecular weight was assayed by SDS-PAGE method. Yeast isolation and identification from Sumbawa mare milk found Saccharomyces cerevisiae. Secretory protein then tested against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 showed activity against Staphylococcus aureus ATCC 25923 about 8.167 mm, meanwhile antimicrobial acitivity against Escherichia coli ATCC 25922 about 6 mm. Each samples found protein with molecular weight about 21,5 kDa, except SKS3. It may be possible that protein with 21,5 kDa play a role in secretory protein’s activity against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923. Secretory protein produced by Saccharomyces cerevisiae more sensitive to Gram positive bacteria than Gram negative bacteria.

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