DENDE MARSI RAHANTO, 051311133017 (2018) UJI AKTIVITAS ANTIMIKROBA Curcuma heyneana DARI JAWA TENGAH DAN DARI MADURA TERHADAP PERTUMBUHAN BAKTERI Bacillus subtillis, Staphylococcus aureus, Escherichia coli DAN JAMUR Candida albicans. Skripsi thesis, UNIVERSITAS AIRLANGGA.
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Abstract
The aim of this study is to determine the antibacterial and antifungal activity of Curcuma heyneana extract from two different areas (temu giring from Central Java and temu late from Madura) against Staphylococcus aureus, Bacillus subtillus, Escherichia coli and Candida albicans. Testing of bacterial activity using agar diffusion and agar dilution methods. Macroscopic observations show that the inner color of the rhizome of the temu giring is yellow and the temu late is brownish yellow. Microscopic observations of cross-sectional rhizome show that the anatomical arrangements of temu giring and temu late are not much different. The yield of essential oil of temu giring and temu late rhizomes is 0.4 % (v/b) and 2 % (v/b) respectively. The TLC test was performed for identification of terpenoid compounds, and showed that in extracts of temu giring and temu late contained terpenoid compounds, the color spot of temu late is more intensive than temu giring. Antibacterial and antifungal activity on diffusion test, showed that the extract of temu late at concentration of 250 μg/well obtained inhibition zone, Staphylococcus aureus 16,98 mm; Bacillus subtillis 15.2 mm; Escherichia coli 14.3 mm; and 8.76 mm Candida albicans fungus. Meanwhile, the extracts of temu giring does not have inhibition zone at the concentration of 250 μg/well. The dilution test showed that temu late extract had MIC in Staphylococcus aureus at 625 ppm, Bacillus subtillis at 1250 ppm, Escherichia coli at 1250 ppm. In conclusion temu giring has no antibacterial and antifungal activity and temu late has the antibacterial and anti-fungal activity at concentration of 250 μg/well.
| Item Type: | Thesis (Skripsi) | |||||||||
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| Additional Information: | KKB KK-2 FF.FT. 02-18 Rah u | |||||||||
| Uncontrolled Keywords: | Curcuma heyneana, Antimicrobial activity, Bacillus subtillis, Staphylococcus aereus, E. coli, and Candida albicans. | |||||||||
| Subjects: | R Medicine > RS Pharmacy and materia medica > RS1-441 Pharmacy and materia medica | |||||||||
| Divisions: | 05. Fakultas Farmasi > Farmakognosi Fitokimia | |||||||||
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| Depositing User: | Tatik Poedjijarti | |||||||||
| Date Deposited: | 18 Mar 2018 19:23 | |||||||||
| Last Modified: | 18 Mar 2018 19:23 | |||||||||
| URI: | http://repository.unair.ac.id/id/eprint/70944 | |||||||||
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