Agung Sosiawan and Didik Raharjo and Indah Nuraini and Nadia Kartika and Alexander Patera Nugraha and Muhammad Dimas (2018) Detection of short tandem repeats at 5 loci and amelogenin with cell-free fetal DNA as a specimen in the development of prenatal paternity diagnostic tests. egyptian Jurnal of Forensic Sciences, 8 (15). pp. 1-3. ISSN 2090-5939

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Abstract

lntroduction: A prenatal paternity lest is one widely-used method of determining the paternity of an unborn child. Such tests using chorionic villus or amniocentesis may increase the risk of harm to both mother and foetus. ln the presenr day, a [renatal paternity test using circulating cell-free fetal Deoxyribonucleic Acid (DNA) is one alternative method due to it being non-invasive and safe for both mother and foetus. Aim: The aim of this study is to detect Short Tandem Repeat (STR) at 5 Loci (vWA, TH01, Dl3S317, Dl8551, and D21 51 1) and amelogenin genes in circulating cell-free fetal DNA in paternity tests. Materials and methods: Forty-one samples of maternal blood were obtained from pregnant woman. circulating free fetal DNA was subsequently egracted. A paternity test was conducted using an SfR test at loci vWA TH01. Dl35312 Dl8S5l, D2'tS11 in circulating free fetal DNA An analysis of the paternity test between loci > 200 bp and < 200 bp was also conducted to establish the sensitivity of the test Results: There was a significant difference bewveen maternal blood DNA and circulating cell-free feUl DNA (o = 0.000 D13S3i7; p=O.OOO D21511; p=0.000 D18S51;p=0.000 vWA; p=0.000 THO1;and p=0.000 amelogenin genes). The locus < 200 bp also had a higher sensitivity than locus > 200 bp. Conclusion: Circulating free fetal DNA can be used as an alternative sample for prenatal paternity tests because of its similarity with maternal DNA. Keywords: Early detection, Circulating free fetal DNA, Non-invasive technique, Prenatal paternity test

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