Isolation and Identification of Transforming Growth Factor β from In Vitro Matured Cumulus Oocyte Complexes

Widjiati and Boediono, Arief and Sumitro, Sutiman Bambang and Aucky, Hinting and Aulanni'am, - and Susilowati, Trinil (2012) Isolation and Identification of Transforming Growth Factor β from In Vitro Matured Cumulus Oocyte Complexes. Hayati Journal of Biosciences, 19 (1). p. 5. ISSN 1978-3019

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Abstract

Transforming growth factor-β (TGF-β) is a two-chain polypeptide with molecular weight of 25 kDa which takes significant role in the steroidogenesis process. In the ovarian oocyte in particular, TGF-β has an important role in regulating reproductive function. TGF-β represents a key intrafollicular protein that regulates follicle development and aromatization process. The purpose of this research was to characterize and identify a protein fraction of TGF-β from the bovine isolated oocytes, which is synthesized during in vitro oocyte maturation process. Oocytes were collected from follicles with diameter of 3-8 mm. Oocytes were then matured in TCM 199 media supplemented with 5 μg/mg LH, 3% BSA, and 50 μg/ml gentamicin sulfate, and cultured in CO2 incubator (5%, 38.5 oC) for 20 hours. TGF-β receptors were identified immunohistochemically. Characteristics of the TGF-β protein were determined using SDS PAGE and TGF-β specification was tested using Western Blotting. The results showed that TGF-β receptors were identified and found in cumulus oocyte complexes (COCs). TGF-β protein was isolated from bovine oocytes with molecular weight 25 kDa and it was identified by Western blotting methods in the same molecular weight

Item Type: Article
Uncontrolled Keywords: transforming growth factor-β (TGF-β), cumulus oocyte complexes (COCs), identification
Subjects: S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine
S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine > Including veterinary genetics, ethology, anatomy, physiology, embryology, pathology
Divisions: 06. Fakultas Kedokteran Hewan > Anatomi Veteriner
Creators:
CreatorsNIM/NIDN
WidjiatiUNSPECIFIED
Boediono, AriefUNSPECIFIED
Sumitro, Sutiman BambangUNSPECIFIED
Aucky, HintingUNSPECIFIED
Aulanni'am, -UNSPECIFIED
Susilowati, TrinilUNSPECIFIED
Depositing User: Nn Erna Dwi Indriyani
Date Deposited: 23 May 2018 21:20
Last Modified: 01 Aug 2018 17:11
URI: http://repository.unair.ac.id/id/eprint/72094
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