EWIK FITRI PRISTYA NINGRUM, 061411131140 (2018) PENGARUH BERBAGAI KONSENTRASI BOVINE SERUM ALBUMIN PADA KULTUR IN VITRO EMBRIO MENCIT (Mus musculus) TERHADAP JUMLAH BLASTOSIS. Skripsi thesis, Universitas Airlangga.

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Abstract

This research was aimed to know the best concentration of bovine serum albumin for increasing the number of blastocyst. Oocyte was obtained from Balb / C mice. The ova were collected and then classified into 3 groups : P1 (MEM + BSA 3%), P2 (MEM + BSA 5%), and P3 (MEM + BSA 7%). Semen was collected from the fertile male, then the motile spermatozoa were inserted into medium which has ova in it. Media containing sperm and ova stored in 5% CO2 incubator. The observations were performed under an inverted microscope with 100x magnification. Embryos that developed until the blastocyst stage showed clear cytoplasm, clear plasma membrane, granular presence, and the presence of zona pellucida. Based on statistical data analysis, it shows significant difference between the highest and lowest concentration of p<0.05, with the most significant different P1 group (54.00a±8,94) and P3 group (32.00b±10,95). In conclusion, the optimal BSA concentration in supporting embryonic development is BSA 3%.

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