LILIK HERAWATI, NIM011417017317 (2018) MEKANISME PENGARUH PREKONDISI DIET GLUKOSA SECARA KONTINYU-BERTAHAP TERHADAP EKSPRESI AKT, PDX1, HSP27, DAN INSULIN SEL BETA PANKREAS YANG DIPAPAR DIET TINGGI GLUKOSA. Disertasi thesis, Universitas Airlangga.

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Abstract

AIM: This study was to figure out the mechanism of the effect of progressive-continuous glucose precondition diet on the expression of Akt, Pdx1, Hsp27, insulin beta cells, beta cell density, serum insulin level, and blood glucose level in high-glucose diet subject. METHOD: White male balb/c mice (Mus musculus), about 5 weeks old were divided on three groups (control, ‘precondition control’, and precondition). The sample size was 9 per group. The administration of moderate glucose precondition diet was for 4 weeks and then got the stressor high glucose diet for 4 weeks on precondition groups. The ‘precondition control’ received standar diet for 4 weeks and stressor high glucose diet for next 4 weeks. Expression of Akt, Pdx1, Hsp27, insulin beta cells, beta cell density, serum insulin level, and blood glucose level were analyzed. RESULTS: Compared with the other groups, precondition groups had elevated blood glucose level and decreased serum insulin level (p = 0.021; p = 0.002), however the lowest of serum insulin level was in ‘precondition control’ group. The Hsp27 expression showed a decrease in the precondition group compared with the other groups (p = 0.043). Whereas the expression of Akt, Pdx1, beta cell insulin, and beta cell density showed no significant difference (p 0.05). CONCLUSION: Moderate-dose glucose precondition diet reveals that the precondition glucose diet may have better functional effect on glucose metabolism, through the Hsp27 expression, however it needs futher investigation to uncover the mechanism of other mediators involved in beta cells function.

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