The role of heat shock protein 27 (HSP 27 ) as inhibitor apoptosis in hypoxic conditions of bone marrow stem cell culture

Sri Wigati Mardi, Mulyani and Ernie Maduratna, Setiawati and Erma, Safitri and Eha Renwi, Astuti (2014) The role of heat shock protein 27 (HSP 27 ) as inhibitor apoptosis in hypoxic conditions of bone marrow stem cell culture. Majalah Kedokteran Gigi, 47 (1). pp. 41-44. ISSN l978-3728

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Official URL: https://e-journal.unair.ac.id/MKG

Abstract

Background: The concept of stem cell therapy is one of the new hope as a medical therapy on salivary gland defect. However, the lack of viability of the transplanted stem cells survival rate led to the decrease of effectiveness of stem cell therapy. The underlying assumption in the decrease of viability and function of stem cells is an increase of apoptosis incidence. It suggests that the microenvironment in the area of damaged tissues is not conducive to support stem cell viability. One of the microenvironment is the hypoxia condition. Several scientific journals revealed that the administration of hypoxic cell culture can result in stress cells but on the other hand the stress condition of the cells also stimulates heat shock protein 27 (HSP 27) as antiapoptosis through inhibition of caspase 9. Purpose: The purpose of this study was to examine the role of heat shock protein 27 as inhibitor apoptosis in hypoxic conditions of bone marrow stem cell culture. Methods: Stem cell culture was performed in hypoxic conditions (O2 1%) and measured the resistance to apoptosis through HSP 27 and caspase 9 expression of bone marrow mesenchymal stem cells by using immunoflorecence and real time PCR. Results: The result of study showed that preconditioning hypoxia could inhibit apoptosis through increasing HSP 27 and decreasing level of caspase 9. Conclusion: The study suggested that hypoxic precondition could reduce apoptosis by increasing amount of heat shock protein 27 and decreasing caspase 9.

Item Type: Article
Uncontrolled Keywords: Mesenchymal stem cells, hypoxia, HSP 27, caspase-9, apoptosis
Subjects: R Medicine
R Medicine > RK Dentistry
Divisions: 02. Fakultas Kedokteran Gigi > Periodontics
Creators:
CreatorsNIM/NIDN
Sri Wigati Mardi, MulyaniUNSPECIFIED
Ernie Maduratna, SetiawatiUNSPECIFIED
Erma, SafitriUNSPECIFIED
Eha Renwi, AstutiUNSPECIFIED
Depositing User: Rudy Febiyanto
Date Deposited: 18 Jul 2018 18:20
Last Modified: 18 Jul 2018 18:20
URI: http://repository.unair.ac.id/id/eprint/73100
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