Phase Behavior of Dried-DDA Liposomal Formulation Dispersed in HPMC Matrix in the presence of Saccharides

Yusuf, Helmy and Nugraheni, Raditya and Mulyadi, Nur Aini and Setyawan, Dwi and Rosita, Noorma (2017) Phase Behavior of Dried-DDA Liposomal Formulation Dispersed in HPMC Matrix in the presence of Saccharides. International Journal of Pharm Tech Research, 10 (1). pp. 50-56. ISSN 0974-4304

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Official URL: http://sphinxsai.com/2017/ph_vol10_no1/ph01.htm

Abstract

The present study describes the effect of saccharides and hydroxypropyl methylcellulose (HPMC) matrix on phase behavior of dehydrated cationic dimethyl- dioctadecylammonium (DDA)-based liposomes. Saccharides such as sucrose, lactose and mannitol,have been reportedpreserve the lipid membranes during drying, whilst HPMC matrix is widely used in solid dispersion to prevent aggregation and/or recrystallization.The study revealed that addition of sucrose and HPMCin the formulation demonstrated a miscible mixture that might construct a stable dried liposomal formulation. DTA data showed that sucrose (5%w/v) and HPMC added to DDA liposomal formulation were relatively more miscible with the mixtures; whereas lactose and mannitol at the same concentration of 5% showed phase separation from the mixtures in the dehydrated state. Furthermore, XRD and SEM analysis exhibited supporting evidences in which formulation using sucrose and lactose showedrelatively less crystalline-forming properties compared to formulation using mannitol. Recrystallization that cause phase separation might trigger leakage and further affect the efficacy of the entrapped drug/antigen. From these data, it might be concluded that a driedliposomal formulation can be prepared in the presence of sucrose (lyoprotectant) that is dispersed in HPMC matrix. The protective mechanism of sucrose (5%w/v) and HPMC matrix is proposed through inhibition of the recrystallization which causes phase separation; indicated by DTA, SEM and XRDdata. The present study revealed prospective advantages of using sucrose and HPMC in development of dried – DDA liposomal formulations. Keywords : Liposomes, DDA, Sucrose, Lactose, Mannitol, HPMC, Phase Separation.

Item Type: Article
Uncontrolled Keywords: Liposomes, DDA, Sucrose, Lactose, Mannitol, HPMC, Phase Separation.
Subjects: R Medicine
R Medicine > RS Pharmacy and materia medica
R Medicine > RS Pharmacy and materia medica > RS1-441 Pharmacy and materia medica
R Medicine > RS Pharmacy and materia medica > RS200-201 Pharmaceutical dosage forms
Divisions: 05. Fakultas Farmasi
Creators:
CreatorsNIM/NIDN
Yusuf, Helmyhelmy-yusuf@ff.unair.ac.id
Nugraheni, RadityaUNSPECIFIED
Mulyadi, Nur AiniUNSPECIFIED
Setyawan, Dwidwisetyawan-90@ff.unair.ac.id
Rosita, Noormanoorma_rosita@ff.unair.ac.id
Depositing User: Mr M. Fuad Sofyan
Date Deposited: 21 Nov 2018 13:11
Last Modified: 21 Nov 2018 13:11
URI: http://repository.unair.ac.id/id/eprint/73336
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