Effect of Caffeine on Morphology of Epididymis Spermatozoa of Bali Bull

Putranti, Oktora D and Soeparna, - and Lestari, Tita Damayanti and Adriani, Lovita (2015) Effect of Caffeine on Morphology of Epididymis Spermatozoa of Bali Bull. IPB Bogor, Bogor - Indonesia, pp. 269-271. ISBN 978-602-96530-4-5

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Abstract

There is an unexpected loss of breeding animals of high genetic value for any reason, such as loss of libido, reproductive tract injury, or death. This loss can be reduced by harvesting spermatozoa from the epididymis with cryopreservation. However, after cryopreservation, there is still a decline of 50% in sperm post-thaw viability after thawing process. This research was conducted to evaluate the use of caffeine to improve cryopreservation quality of Bali bull’s spermatozoa. Selection for sperm quality was based on sperm morphology. Cauda epididymis was obtained from slaughterhouse and the semen was collected and examined with caffeine after cryopreservation. The spermatozoa were stained with Acredine Orange by microscopy laser-scanning confocal microscope (BioRad MRC-1024). The result demonstrated that control have high value of normal sperm cell than those treated with caffeine. Key words: cauda epididymis, abnormality, caffeine

Item Type: Book
Uncontrolled Keywords: cauda epididymis, abnormality, caffeine
Subjects: S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine
Divisions: 06. Fakultas Kedokteran Hewan > Reproduksi Veteriner
Creators:
CreatorsNIM/NIDN
Putranti, Oktora DUNSPECIFIED
Soeparna, -UNSPECIFIED
Lestari, Tita DamayantiUNSPECIFIED
Adriani, LovitaUNSPECIFIED
Depositing User: Nn Erna Dwi Indriyani
Date Deposited: 03 Oct 2018 11:03
Last Modified: 03 Oct 2018 11:03
URI: http://repository.unair.ac.id/id/eprint/74082
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