PENAPISAN DAN IDENTIFIKASI BAKTERI PENGHASIL ENZIM FIBRINOLITIK PADA PETIS

MAHARANI PUSPITA SARI, 051411131044 (2018) PENAPISAN DAN IDENTIFIKASI BAKTERI PENGHASIL ENZIM FIBRINOLITIK PADA PETIS. Skripsi thesis, Universitas Airlangga.

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Abstract

Petis is one of the fermentation product which is made with marine creatures such as fish or shrimp. A fibrinolytic in some fermentation food from marine creatures were discovered. Screening of bacteria that produce fibrinolytic enzyme using six petis from different home industry. The isolation of samples was obtained from Skim Milk Agar proteolytic test and fibrin plate test. Identification of the bacteria performed with gram staining test and sequencing 16S rRNA method. The sample was diluted with NaCl 0,9% up to 10-7 then inoculated on the media SMA and incubated in 37ºC for 24 hours. All samples have the proteolytic activity that shown by a clear zone around the colony. There were seventeen proteolytic isolates bacteria tested fibrinolytic activity with fibrin plate then incubated in 37ºC for 24 hours. The seventeen isolates showed positive result to fibrinolytic activity with highest fibrinolytic index was P3c. Isolate bacteria P3c was identified using sequencing gene 16s rRNA. The result of identifying, the bacteria that have strong fibrinolytic activity was Bacillus flexus.

Item Type: Thesis (Skripsi)
Additional Information: KKB KK-2 FF.KF. 08-18 Sar p
Uncontrolled Keywords: petis, screening, identification, fibrinolytic enzyme, Bacillus flexus.
Subjects: Q Science > QR Microbiology > QR75-99.5 Bacteria
R Medicine > RS Pharmacy and materia medica > RS1-441 Pharmacy and materia medica
Divisions: 05. Fakultas Farmasi > Kimia Farmasi
Creators:
CreatorsEmail
MAHARANI PUSPITA SARI, 051411131044UNSPECIFIED
Contributors:
ContributionNameEmail
ContributorA. Toto Poernomo, Dr., M.Si.,Apt.UNSPECIFIED
ContributorAmirudin Prawita, Prof. Dr., Apt.UNSPECIFIED
Depositing User: Tatik Poedjijarti
Date Deposited: 12 Dec 2018 12:42
Last Modified: 12 Dec 2018 12:42
URI: http://repository.unair.ac.id/id/eprint/76629
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