SKRINING DAN IDENTIFIKASI BAKTERI PENGHASIL ENZIM FIBRINOLITIK DARI TAUCO

ENI SETYAWATI, 051411133031 (2018) SKRINING DAN IDENTIFIKASI BAKTERI PENGHASIL ENZIM FIBRINOLITIK DARI TAUCO. Skripsi thesis, Universitas Airlangga.

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Abstract

Tauco is one kind of traditional fermented food from Indonesia. Tauco is processed from soybean paste that have salty taste and commonly have yellow and black colour. Generally, tauco also used as spices or seasonings in various daily dishes. The aim of the study is to find and identified the bacteria that produced fibrinotic enzymes in tauco. The sample used in this research is tauco from six different manufacturers. There are two tests that have to do in this study, that are proteolytic test of Agar Milk Scale and fibrin plate test. Both of that tests is used to gained the bacteria producing fibrinotic enzymes. Bacteria is identified macroscopically, gram staining and 16S rRNA sequencing. In the proteolytic test the sample was diluted with 0.9% up to 10-7 NaCl then inoculated on Agar Milk Skim medium, incubated at 37ºC for 24 hours. All of samples have proteolitic activity that shown by the formation of clear zones around bacterial colonies. A total of 17 isolates of proteolytic bacteria were incubated at 37ºC for 24 hours and then used for the fibrin plate test. All of isolate proteolitic bacteria produces fibrinolytic activity with the largest fibrinolytic index in TC4a isolate of 3,46. Furthermore, TC4a bacteria were genotypically identified by sequencing the 16S rRNA gene. According the result of tauco identification, is found that the bacteria that strongly produced fibrinotic enzymes in tauco sample TC4a is similar with Bacillus cereus TC4a

Item Type: Thesis (Skripsi)
Additional Information: KKB KK-2 FF.KF. 46- 18 Set s
Uncontrolled Keywords: Fibrinolytic enzyme, Fibrinolytic activity, Tauco, Screening, Identification, 16s rRNA, Bacillus cereus TC4a
Subjects: Q Science > QR Microbiology > QR75-99.5 Bacteria
R Medicine > RS Pharmacy and materia medica > RS1-441 Pharmacy and materia medica
Divisions: 05. Fakultas Farmasi > Kimia Farmasi
Creators:
CreatorsEmail
ENI SETYAWATI, 051411133031UNSPECIFIED
Contributors:
ContributionNameEmail
ContributorA. Toto Poernomo, Drs., M.Si., Apt.UNSPECIFIED
ContributorSudjarwo, Prof. Dr.. M.S., Apt.UNSPECIFIED
Depositing User: Tatik Poedjijarti
Date Deposited: 13 Dec 2018 18:07
Last Modified: 13 Dec 2018 18:07
URI: http://repository.unair.ac.id/id/eprint/76744
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