EKA FITRIA AUGUSTINA, NIM011417017309 (2019) MEKANISME PERBAIKAN EPITEL GINGIVA TERHADAP PAPARAN LPS PORPHYROMONAS GINGIVALIS MELALUI EKSPRESI CLAUDIN-1, ECADHERIN, TLR-4, NFκB, COX-2, PGE2, DAN MMP-7 DENGAN PEMBERIAN KURKUMIN( Studi Eksperimental pada Hewan coba Rattus norvegicus Model Gingivitis ). Disertasi thesis, Universitas Airlangga.

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Abstract

Background : Inflammatory disease of periodontal tissue (periodontitis) is still a problem in many developing countries including in Indonesia due to its very high prevalence.The disease is an infectious inflammatory condition of the periodontal tissue caused by Porphyromonas gingivalis. Gingival epithelial plays an important role in oral tissue protection from invasion or bacterial attack. Epithelial cells act as mechanical barriers to the attack of pathogenic organisms and enhance intercellular communication through complex cell-cell junction. Administration of Curcumin is expected to increased epithelial defences. Purpose : The purpose of this study was to prove the role of Claudin-1, E-Cadherin, TLR-4, NFκB, COX-2, PGE-2, and MMP-7 in barrier epihelial in gingiva epithelium. Furthermore, recent study to examine the influence of curcumin to increased barrier epihelial in gingiva epithelium and repair the gingival epithelium damaged by exposure to LPS bacteria Pg. Methods : The experimental animals used were white rats (Rattus norvegicus), Wistar strains, males, 12 weeks old, weighing 200-250 grams. All animals adapted for 2 weeks. Then the random allocation of 40 rats divided into 8 time-based research groups. One as a healthy group and the seven others were exposed by LPS Pg. Six groups consist of three which injection by LPS Pg and three treatment groups which treatment with curcumin 1%. The other one group were control for the vehicle. Sample was taken from gingival tissue of mandibula incisors. On 1 day, 7 days and 14 days, Claudin-1, E-Cadherin, TLR-4, NFκB, COX-2, PGE-2, and MMP-7 expression were examinated by immunochemistry method. Tight junctions and adherens junction bonds were examined by Transmission Electron Microscopy (TEM). Results and discussion : In groups which injected by LPS Pg, showed an increase in TLR-4, NFκB, COX-2, PGE-2, and MMP-7 expression and decreased Claudin-1 and E-Cadherin expression (p<0,05). Administration of 1% curcumin topically can reduced TLR-4, NFκB, COX-2, PGE-2, and MMP-7 expression and increased Claudin-1 and E-Cadherin expression (p<0,05). Examination with TEM showed the bonds of tight junction (TJ) and adherent junction (AJ) in the treatment group occurred with LPS Pg showed tissue damage ie visible cell swelling, cell shrinking, and disruption of TJ and AJ. Examination with TEM showed that the bonds of tight junction (TJ) and adherent junction (AJ) in LPS Pg group with curcumin administration showed tissue repair that showed start intact cell. In this study, LPS Pg can cause decreased expression of tight junction protein, Claudin-1 and adherent junction protein, E-Cadherin. This decrease causes the tight junction and adherent junction damage that is visible from the TEM image. Administration of curcumin may increase Claudin-1 and E-Cadherin expression resulting in improved tight junction and adherent junction bonds, as seen from the TEM Conclusion : The conclusions of this study were, Claudin-1, E-Cadherin, TLR-4, NFκB, COX-2, PGE-2, and MMP-7 play an important role in barrier epithelial in gingiva epithelium. Administration of 1% curcumin topically can repair the gingival epithelium damaged by exposure to LPS bacteria Pg.

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