UJI AKTIVITAS BIOSURFAKTAN SUPERNATAN KALDU FERMENTASI ISOLAT BAKTERI ASAL SEDIMEN MANGROVE WONOREJO SURABAYA DAN POTENSINYA SEBAGAI ANTIJAMUR TERHADAP Candida albicans ATCC 10231

BIMA WIDYA PRAMUDIANTO, 061511133120 (2019) UJI AKTIVITAS BIOSURFAKTAN SUPERNATAN KALDU FERMENTASI ISOLAT BAKTERI ASAL SEDIMEN MANGROVE WONOREJO SURABAYA DAN POTENSINYA SEBAGAI ANTIJAMUR TERHADAP Candida albicans ATCC 10231. Skripsi thesis, Universitas Airlangga.

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Abstract

The purpose of this research was to know the genus of bacterial isolate, biosurfactant activity of bacterial isolate, and potency biosurfactant as antifungal against Candida albicans ATCC 10231. Bacterial isolate from mangrove sediments of Wonorejo Surabaya. Mangrove Wonorejo Surabaya is known contaminated of heavy metals and hidrocarbon. Heavy metals and and hidrocarbon contaminant could induce microorganism to produce biosurfactant. Biosurfactant have amphiphilic characteristic that can reduce the surface tension. Because of that biosurfactant have been to use for therapeutic and biomedical importance, such as antibacterial, antifungal and antiviral properties. Biosurfactant activity test used oil spreading, drops collapse, and parafilm test and antifungal activity test used agar well diffusion method. The result of this research showed that Genus of bacterial isolate belong to Bacillus because the bacterial isolate was Gram positive, bacil shape, aerob, and having endospore with ellipse shape which was in center of the cell. The result of biosurfactant activity with oil spreading method showed by clear zone. The average clear zone was 48.88 mm, the average droplets of parafilm test is 6.41 mm, and the result drops collapse test is positive showed by spread and drowned cell free supernatant of bacterial isolate. Cell free supernatant of bacterial isolate could inhibit growth of Candida albicans. The average inhibitory diameter is 31.25 mm. The conclusion of this research proved that bacterial isolate belong to Genus Bacillus which have biosurfactant and antifungal of Candida albicans. Keyword: Biosurfactant, Antifungal, Candida albicans

Item Type: Thesis (Skripsi)
Additional Information: KKC KK KH. 65/19 Pra u
Uncontrolled Keywords: Biosurfactant, Antifungal, Candida albicans
Subjects: Q Science > QR Microbiology > QR75-99.5 Bacteria
Divisions: 06. Fakultas Kedokteran Hewan
Creators:
CreatorsEmail
BIMA WIDYA PRAMUDIANTO, 061511133120UNSPECIFIED
Contributors:
ContributionNameEmail
ContributorSuryanie Sarudji, drh., M.Kes.UNSPECIFIED
ContributorRahmi Sugihartuti, Dr., drh., M.Kes.UNSPECIFIED
Depositing User: sukartini sukartini
Date Deposited: 01 Mar 2019 03:33
Last Modified: 01 Mar 2019 03:33
URI: http://repository.unair.ac.id/id/eprint/80637
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