MIEKE KUSUMA DEWI, 021628036321 (2018) VIABILITAS SEL FIBROBLAS PULPA SETELAH APLIKASI KALSIUM HIDROKSIDA, PROPOLIS, DAN KOMBINASI KALSIUM HIDROKSIDA-PROPOLIS. Thesis thesis, Universitas Airlangga.

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Abstract

Background Calcium hydroxide have shown the best results, since they favor repair of the injured pulp tissue and stimulate the formation of a hard tissue barrier, but however, that the high pH generated by calcium hydroxide is potentially toxic to pulp tissue and causing cell necrosis. It can cause “ Tunnel defect” formation. Calcium Hydroxide combined propolis had good biocompatibility in fibroblast cell. Aim: This study aim to compare the in vitro viability of fibroblast pulp cell following application combination of Calcium Hydroxide and propolis. Method: In this in vitro study, the culture cell divided into 7 groups: Group 1 - calcium hydroxide 10 μg; Group 2 – propolis 10 μg; Group 3 propolis 15 μg; Group 4 propolis 20 μg; Group 5 calcium hydroxidepropolis 1:1; Group 6 calcium hydroxide-propolis 1:1,5; Group 7 calcium hydroxide-propolis 1:2. After the calcium hydroxide, propolis and the combine were applied in culture cell, then placed in incubator for 24 hours. The methylthiazolyl diphenyl-tetrazolium bromide (MTT) was carried out to eavluate the viability of fibroblast pulp cell. Result: All sample were examined and data was statiscally analysed using Oneway Anova and following Tukey-HSD. Comparison of cell viability showed that cells subjected to combination of calcium hydroxide-propolis were more viable when compared to calcium hydroxide and propolis (P < 0.05). Conclusion: application combination of calcium hydroxide and propolis extract have better biocompatibility in fibroblast cell viability rather than calcium hydroxide or propolis alone.

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