Pengaruh Penyuntikan Isolat Prolaktin Itik Fase Moulting Terhadap Timbulnya Antibodi Poliklonal Anti Prolaktin Kambing

Hariadi, Mas'ud and Rizqi, Resmita Citra and Hidanah, Sri (2009) Pengaruh Penyuntikan Isolat Prolaktin Itik Fase Moulting Terhadap Timbulnya Antibodi Poliklonal Anti Prolaktin Kambing. Journal of Poultry Science, 2 (1). pp. 34-39. ISSN 1979-7222

[img] Text (Jurnal)
Bukti C10 Pengaruh Penyuntikan Isolat ....-compressed.pdf

Download (1MB)
[img] Text (Peer Review)
Peer Review Bukti 10.pdf

Download (1MB)

Abstract

An experiment was conducted to produce anti prolactin polyclonal antibody and get the highest optical density (OD) result after injection isolate prolactin goat. Raising anti prolactin polyclonal antibody used six male local goats, five male local goats injected with 275 µg/ml isolate prolactin in Complete Freund’s Adjuvant (CFA) and one male local goat injected with PBS as a control. The first booster (repeat injection) had done with 275 µg/ml isolate prolactin in Incomplete Freund’s Adjuvant on the 12th days. One day before the first injection and every week after the first booster until the 13th booster (the last booster) did bleed two times. ELISA indirect test was perform the appearance anti prolactin polyclonal antibody as well as the highest OD result. ANOVA test (Analysis of Variance) was apply in order to identifty the difference among dose treatments then continue using Honestly Significant Difference test (5%). These results anti prolactin polyclonal antibody was appear at first bleeding and the highest OD antibody polyclonal anti prolactin in the 17th bleeding (1,00467 ± 0,47112). The conclusion strongly indicated anti prolactin polyclonal antibody for duck could be produce from the goat with OD (1,00467 ± 0,47112). Keywords : duck, goat, moulting, prolactin, polyclonal antibody

Item Type: Article
Uncontrolled Keywords: duck, goat, moulting, prolactin, polyclonal antibody
Subjects: S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine
S Agriculture > SF Animal culture > SF600-1100 Veterinary medicine > SF811-909 Veterinary medicine of special organs, regions, and systems
Divisions: 06. Fakultas Kedokteran Hewan > Ilmu Peternakan
Creators:
CreatorsNIM/NIDN
Hariadi, Mas'udUNSPECIFIED
Rizqi, Resmita CitraUNSPECIFIED
Hidanah, SriUNSPECIFIED
Depositing User: Nn Erna Dwi Indriyani
Date Deposited: 21 Jun 2019 11:15
Last Modified: 25 Jun 2019 08:19
URI: http://repository.unair.ac.id/id/eprint/83211
Sosial Share:

Actions (login required)

View Item View Item