PENGARUH INTENSITAS CAHAYA TERHADAP BIOMASSA DAN KADAR SAPONIN KALUS GINSENG JAWA (Talinum paniculatum Gaertn.) PADA BERBAGAI WAKTU KULTUR

Afifatul Alwiyah and Yosephine Sri Wulan Manuhara and Edy Setiti Wida Utamy (2014) PENGARUH INTENSITAS CAHAYA TERHADAP BIOMASSA DAN KADAR SAPONIN KALUS GINSENG JAWA (Talinum paniculatum Gaertn.) PADA BERBAGAI WAKTU KULTUR. Jurnal Ilmiah Biologi, 2 (1). pp. 56-66. ISSN 9 772303 34-2002

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Abstract

Abstract The aims of this research was to study the effects of light intensity on callus biomass and saponin content of ginseng jawa (Talinum paniculatum Gaertn.). Callus induced from leaf explants on solid Murashige and Skoog (MS) medium supplemented by 2 mg/L of 2,4-D and 1 mg/L of kinetin. Callus induced by treatment of combination between light intensities 1000, 2000, 4000, 6000 lux, also dark condition and culture period 4, 6, and 8 weeks. Each treatment of combination was repeated 6 times. Retrieval of data were callus biomass (fresh and dry weight) and saponin content. The data of biomass were analyzed by using manova test (significance of 5%). Saponin content was analyzed semi-quantity using Thin Layer Chromatography (TLC). The result showed that the highest average of dry weight (as parameter) obtained at treatment combination 4000 lux light intensity and 8 weeks culture period was 0,0699 gram. Meanwhile the highest saponin content obtained at 4000 lux light intensity and 6 weeks culture period was 58,5 mm 2/0,01 gram dry weight of callus and color intensity +4. With the results that light intensities influence on callus biomass and saponin content of ginseng jawa at different culture period.

Item Type: Article
Uncontrolled Keywords: biomass, callus, culture period, light intensity, saponin content, Talinum paniculatum Gaertn
Subjects: Q Science > Q Science (General)
Divisions: 08. Fakultas Sains dan Teknologi > Biologi
Creators:
CreatorsNIM/NIDN
Afifatul AlwiyahUNSPECIFIED
Yosephine Sri Wulan ManuharaUNSPECIFIED
Edy Setiti Wida UtamyUNSPECIFIED
Depositing User: Mr Akhmad Nurfizal Reza
Date Deposited: 25 Jun 2019 07:55
Last Modified: 25 Jun 2019 07:55
URI: http://repository.unair.ac.id/id/eprint/83554
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