Agrobacterium rhizogenes Mediated Hairy Root Induction in Justicia gendarussa Burm.f

Dwi Kusuma Wahyuni and Febri Vidianti and Hery Purnobasuki and Tri Muji Ermayanti and Bambang Prajoga and Edy Setiti Wida Utami (2015) Agrobacterium rhizogenes Mediated Hairy Root Induction in Justicia gendarussa Burm.f. Journal of Applied Environmental and Biological Sciences, 5 (4). pp. 87-93. ISSN 2090-4274

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Official URL: https://www.researchgate.net/publication/274640729...

Abstract

ABSTRACT Five strains of Agrobacterium rhizogenes (LB510, LB509, YMB072001, A4T, and ATCC 15834) were tested to determine their effects on the induction of hairy root on leaf explants of Justicia gendarussa Burm. f.. Leaf explants were sterilized, and then infected within liquid MS medium + sucrose containing A. rhizogenes OD600 = 0.1, for 20 minutes while being shaken gently by hand. Teh explant was co-cultivated on Murashige and Skoog (MS) solid medium at 25°C for 48 hours. After 48 hours of co- cultivation, explants were transferred to MS solid medium with the addition of 250 mg/L cefotaxim, and repeated 1-2 times. Observations were carried out during 6 weeks to observe the specified parameters. Data were analized by Kruskal-Wallis Test and followed by Mann-Whitney Test. All strains of A. rhizogenes were used in this study were able to produce hairy root. The treatment of LB510 and YMB072001 strain was the best strain in the hairy root induction of J. gendarussa Burm. f.. The infection frequency of LB510 strain was highest ( 100 % ), longest hairy root (2.23 cm), and highest of hairy root wet weight (0.0243 g). The treatment of YMB072001 strain was the highest number of hairy roots (3.3) per explant and the highest number of hairy roots dry weight (0.0098 g).

Item Type: Article
Uncontrolled Keywords: Agrobacterium rhizogenes, hairy root, Justicia gendarussa Burm. f.
Subjects: Q Science > Q Science (General)
Divisions: 08. Fakultas Sains dan Teknologi > Biologi
Creators:
CreatorsNIM/NIDN
Dwi Kusuma WahyuniUNSPECIFIED
Febri VidiantiUNSPECIFIED
Hery PurnobasukiUNSPECIFIED
Tri Muji ErmayantiUNSPECIFIED
Bambang PrajogaUNSPECIFIED
Edy Setiti Wida UtamiUNSPECIFIED
Depositing User: Mr Akhmad Nurfizal Reza
Date Deposited: 25 Jun 2019 03:37
Last Modified: 25 Jun 2019 03:37
URI: http://repository.unair.ac.id/id/eprint/83568
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