PERAN ERYTHROPOIETIN DALAM MEMPERLAMBAT PENIPISAN LAPISAN SEL PUNCA ENDOGEN ZONA SUBVENTRIKULAR MELALUI HAMBATAN AKTIVASI MIKROGLIA DAN ASTROSIT REAKTIF PADA HIDROSEFALUS

WIHASTO SURYANINGTYAS, NIM011317017310 (2019) PERAN ERYTHROPOIETIN DALAM MEMPERLAMBAT PENIPISAN LAPISAN SEL PUNCA ENDOGEN ZONA SUBVENTRIKULAR MELALUI HAMBATAN AKTIVASI MIKROGLIA DAN ASTROSIT REAKTIF PADA HIDROSEFALUS. Disertasi thesis, Universitas Airlangga.

[img] Text (Abstrak)
ABSTRACT.pdf

Download (60kB)
[img] Text (Daftar isi)
DAFTAR ISI.pdf

Download (106kB)
[img] Text (daftar pustaka)
DAFTAR PUSTAKA.pdf

Download (148kB)
[img] Text (COVER - BAB 4)
Dis.K.18-19 Sur p (Cover - bab 4).pdf
Restricted to Registered users only until 27 June 2022.

Download (3MB) | Request a copy
[img] Text (BAB 5 - DAPUS)
Dis.K.18-19 Sur p (Bab 5-dapus).pdf
Restricted to Registered users only until 27 June 2022.

Download (2MB) | Request a copy
[img] Text (Lampiran)
Lampiran.pdf
Restricted to Registered users only until 27 June 2022.

Download (1MB) | Request a copy
Official URL: http://lib.unair.ac.id

Abstract

Purpose: To elucidate the potential role of Erythropoietin (EPO) as a neuroprotective agent against reactive strogliosis and reducing the thinning rate of subventricular zone (SVZ) in kaolin-induced hydrocephalic rats. Method: Thirty-six ten-week-old Sprague-Dawley rats were used in this study. Hydrocephalus was induced with 20% kaolin suspension injected into the cistern of thirty rats and leaving the six rats as normal group. The hydrocephalic rats were randomly divided into hydrocephalic and treatment group. The treatment group received daily dose of recombinant human erythropoietin (rhEPO) from day-7 to day-21 after induction. The animals were sacrificed at 7 (only for hydrocephalic group) and 14 or 21 (for both groups) days after induction. Brain was removed and was prepared for histological analysis by hematoxylin and Eosin staining as well as immunohistochemistry for 4-HNE, β-catenin, GFAP, Iba-1 and Ki-67. Results: Immunohistochemical analysis showed that animals treated with rhEPO had a reduced astrocyte reactivity displayed by lower GFAP expression. Hydrocephalic rats received rhEPO also displayed reduced microglial activation shown by lower Iba-1 protein expression. Exogenous rhEPO exerted its protective action in reducing astrogliosis by inhibiting lipid peroxidation that was documented in this study as lower expression of 4-HNE than non-treated group. Expression of β-catenin was also reduced following the pattern of 4-HNE. The SVZ thickness was progressively declining in hydrocephalus group, while the progression rate could be reduced by rhEPO. Conclusion: Erythropoietin inhibited lipid peroxidation, and reactive astrogliosis in hydrocephalic animal model. The reduced thinning rate of SVZ demonstrated that EPO also had effect in reducing the hydrocephalus progressivity. Further research is warranted to explore its efficacy and safety to use in clinical setting.

Item Type: Thesis (Disertasi)
Additional Information: KKA KK Dis.K.18/19 Sur p
Uncontrolled Keywords: Hydrocephalus, Erythropoietin, Subventricular zone, Astrogliosis
Subjects: R Medicine > RC Internal medicine
Divisions: 01. Fakultas Kedokteran
Creators:
CreatorsNIM/NIDN
WIHASTO SURYANINGTYAS, NIM011317017310NIM011317017310
Contributors:
ContributionNameNIDN/NIDK/NUP
ContributorFedik Abdul Rantam, Prof. Dr. , drh.UNSPECIFIED
ContributorArifin Parenrengi, Dr. M. , dr, Sp.BSUNSPECIFIED
Depositing User: Unnamed user with email nafisa@lib.unair.ac.id
Date Deposited: 27 Jun 2019 05:17
Last Modified: 27 Jun 2019 05:17
URI: http://repository.unair.ac.id/id/eprint/84088
Sosial Share:

Actions (login required)

View Item View Item