Tamara Yuanita (2012) Peningkatan Ekspresi Receptor Activator of NF-κB Ligand (RANKL) pada Lesi Periapikal Gigi akibat infeksi bakteri Enterococcus faecalis. Jurnal Conservative Dentistry., 2 (1). pp. 17-21. ISSN 2087-1848.

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Abstract

Background: The Enterococcus faecalis (E faecalis) bacteria is the largest species found in root canal treatment periapical lesions. E faecalis can survive when performed root canal treatment because it can get into the dentin tubuli and firmly attached to the surface area of periapical tooth dentin. Inflammatory bone resorption is a complex process that requires regulation of RANKL (Receptor Activator of NF-κB Ligand) as osteoclast differentiation factor that stimulates osteoclastogenesis cause periapical bone resorption. Purpose: The purpose of this study is to prove by immunohistochemistry increased expression of RANKL in periapical tissues Wistar rats treated with E faecalis bacteria compared to untreated. Methods: Experimental studies on 24 Wistar rats were randomly divided into three groups. Group A is healthy teeth as a negative control. Group B, in the maxillary first molar teeth drilled then put 10.μl BHI then filled with Glass ionomer Cement as a positive control. Group C is the treatment group, after the maxillary first molar teeth drilled then inoculated with 10 μl BHI containing 106 CFU E faecalis bacteria ATCC 29212 and then filled with Glass ionomer Cement to prevent contamination. It takes 21 days to get periapical lesions after pulp infection. Mice were sacrificed and then examination periapical tissue immunohistochemically to see the expression of RANKL. Data were analyzed by ANOVA. Result: The result is an increase in the expression of RANKL in the treatment group (p <0.005) when compared with the negative control group and the positive control group. Conclusion: it can be concluded that the E faecalis bacteria led to increased in RANKL in periapical lesions of teeth.

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