Tatik Hernawati (2016) Supplementation of Specific Proteins in The Seminal Plasma Frozen Semen Diluter Mediaon The Plasma Membrane Intact, The Expression of Caspase and Malondialdehyde Post Thawing. Asian Academic Research Journal of Multidisciplinary, 3 (4). pp. 105-116. ISSN 2319-2801

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	Text (Turn It In) BUKTI 3 SUPPLEMENTATION OF SPECIFIC PROTEINS IN THE SEMINAL PLASMA FROZEN SEMEN DILUTER MEDIAON THE PLASMA MEMBRANE INTACT, THE EXPRESSION OF CASPASE AND MALONDIALDEHYDE POST THAWING-compressed.pdf Download (2MB)

Abstract

Background: The quality of frozen semen after thawing is one of factors in artificial insemination. Cryopreservation affects the post-thawed sperm quality. The seminal plasmadiary bull’s contains high concentration of specific proteinosteopontin. Our hypothesis is that osteopontin maintain sperm quality during cryopreservation. Aim :The aim was to investigate osteopontin does improve the quality of frozen semen as appears from increased The plasma membraneintact (PMI), decreased expression of caspase and Malondialdehyde (MDA) Method : isolation, identification and protein specification of osteopontin from dairy bull semen. using SDS-PAGE method indicated that there were some tapes showing different molecular weight from several samples. SDS-PAGE result from protein identification was continued with protein identification using Western Blot technique. The effect of osteopontinon the quality of frozen semen post thawing by the expression of caspase with immunocy to chemistry techniques and measurement of MDA Result: of the first phase was determined that molecular weight protein specification ofosteopontin is 56 kDa. Osteopontin concentration from electroelution samples were analyzed using Nano drop method, and it showed the protein concentrations as avaregely 180 μg /ml. result of the second phase indicated that the addition of osteopontin can improve semen quality through increased The plasma membraneintact ( PMI), decreased of expression caspase 9 and MDA Conclusion : Addition of osteopontin may improve semen quality through increased The plasma membraneintact ( PMI) decreasedofexpressioncaspase9 and MDA Key words: Spesifik Protein, Friesian Holstein diary bull, seminal plasma, PMI, MDA, Caspase 9

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