In silico and in vivo anti-inflammatory studies of curcuminoids, turmeric extract with zinc oxide, and eugenol

Asti Meizarini and Siswandono and Wibi Riawan and Retno Pudji Rahayu (2018) In silico and in vivo anti-inflammatory studies of curcuminoids, turmeric extract with zinc oxide, and eugenol. Tropical Journal of Pharmaceutical Research. pp. 270-275. ISSN 1596-5996

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Abstract

Purpose: To determine the anti-inflammatory activity of curcuminoids in comparison with that of eugenol in silico, and to determine the anti-inflammatory activity of wound dressings made from zinc oxide powder and liquid turmeric extract with a high curcuminoid content. Methods: In silico studies were conducted, using Molegro Virtual Docker program, to predict the antiinflammatory potency of curcuminoids (curcumin, demethoxycurcumin, bisdemethoxycurcumin) and eugenol against COX-2 receptors. In vivo studies to evaluate anti-inflammatory activity via TNFα expression, were carried out using thirty Wistar rats as subjects, divided into two groups: A (sacrificed on day 3) and B (sacrificed on day 7). Each group contained three subgroups (n = 5): A1, B1 were excised without a dressing as control subgroups; A2, B2 were excised followed by the application of zinc oxide with a turmeric extract dressing; and A3, B3 were excised followed by the application of zinc oxide with an eugenol dressing. Results: The in silico studies confirmed the anti-inflammatory activity of curcumin (-132.905 kcal/mol), demethoxycurcumin (-130.265 kcal/mol), bisdemethoxycurcumin (-118.827 kcal/mol) in relation to the COX-2 receptor to be greater than that of eugenol (-78.718 kcal/mol). The in vivo studies of TNFα expression showed that the levels of activity in the groups without dressings were significantly higher than in those with dressing (p<0.05), while the lowest TNFα expression were for zinc oxide with turmeric extract dressings. Conclusion: The combination of zinc oxide with turmeric liquid extract has a higher anti-inflammatory effect than eugenol as demonstrated by both in vivo and in silico studies. This combination can, therefore, be used as an alternative to zinc oxide eugenol wound dressings.

Item Type: Article
Subjects: R Medicine
R Medicine > RK Dentistry
Divisions: 02. Fakultas Kedokteran Gigi > Patologi & Maxifacial
Creators:
CreatorsNIM/NIDN
Asti MeizariniUNSPECIFIED
SiswandonoUNSPECIFIED
Wibi RiawanUNSPECIFIED
Retno Pudji RahayuUNSPECIFIED
Depositing User: Rudy Febiyanto
Date Deposited: 24 Jul 2019 01:07
Last Modified: 24 Jul 2019 01:07
URI: http://repository.unair.ac.id/id/eprint/85178
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