Ira Arundina and Theresia Indah Budhy S. and Intan Nirwana and Retno Indrawati and Muhammad Luthfi (2016) Toxicity Test n-Hexane : Ethyl Acetate (3:7) Fraction of Sudamala (Artemisia vulgaris L.). Procedia Chemistry, 18. pp. 174-178. ISSN 1876-6196

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Abstract

Sudamala (Artemisia vulgaris L.) is commonly used in the community as anti-tumor in digestive organ, including in oral cavity. However, there have been no scientific studies about oral anti-carcinogenic active substances of Sudamala. The purpose of this study was to analyse the effect of per oral administration of n-hexane : ethyl acetate (3:7) fraction of Artemisia vulgaris L. on healthy mice. This experimental study used male Swiss Webster (Balb C) strain mice (Mus musculus) 2.5 months old, 20-30 grams by weight. Group 1, the mice received 0.5% CMC- Na fraction solvent, as a control. Group 2, the mice received 200 mg/kgbw n-hexane : ethyl acetate (3:7) fraction of Sudamala (Artemisia vulgaris L). The fraction was given once daily for 8 weeks per oral according to the mice's weight. At the end of 8th week, the mice were killed and oral mucosal tissue was taken for biopsy specimens. Tunnel assay for Apoptosis and immunohistochemistry were undertaken for PCNA. T test analysis showed there was no significant difference between control and treatment groups. The fractions of n-hexane : ethyl acetate (3:7) of Sudamala (Artemisia vulgaris L) was not toxic , it was not harmful to living tissue in healthy mice.

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