The differences of effectiveness of β -1,3-glukanase Vigna unguiculata and papain Carica papaya enzymes in hydrolysis of denture plaque

Retno Indrawati, NIDN. 0012115903 and Muhammad Lutfi, NIDN. 0006036704 and Erina Fatmala Yuli Andari (2016) The differences of effectiveness of β -1,3-glukanase Vigna unguiculata and papain Carica papaya enzymes in hydrolysis of denture plaque. Dental Journal (Majalah Kedokteran Gigi), 49 (2). pp. 81-86. ISSN 1978-3728

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Abstract

Background: Accumulation of denture plaque can lead to pathological changes in oral mucosa, such as denture stomatitis, halitosis, and caries. Plaque matrix is mostly formed by protein (30%) and polysaccharide complexes. Thus, an alternative enzyme solution as denture cleanser is required for hydrolysis of denture plaque. Papain is a proteolytic enzyme hydrolyzing proteins, while β-1,3-glucanase is a hydrolase enzyme hydrolyzing polysaccharides. Purpose: This study aimed to analyze the differences of effectiveness of ß-1,3-glucanase Vigna unguiculata enzyme and papain Carica papaya enzyme in hydrolysis of denture plaque. Method: This research was a laboratory experimental research with post test only control group design. After using denture for 24 hours, the denture was soaked in a solution of 100 ml PBS, papain enzyme, and β 1-3 glucanase enzyme at a concentration of 0.5 mg/ml, 1 mg/ml, and 2 mg/ml for 10 minutes. The solution from plaque hydrolysis was soaked in PBS and vortex enzyme for 2 minutes, then soaked in ice water for 15 minutes, and centrifuged at 3000 rpm 5-10º for 10 minutes. The supernatant was separated and analyzed. Turbidity readings then were performed in spectrofotometer with a wavelength of 480 nm. Result: 2 mg/ml of ß-1,3 glucanase enzyme generated the highest values of hydrolysis with a mean percentage of 68.77% compared to papain enzyme (44.86 %). The lowest values of hydrolysis were generated by PBS with a mean percentage of 3.24%. Conclusion: ß-1,3-glucanase enzyme is more effective in hydrolysis of denture plaque than papain enzyme.

Item Type: Article
Subjects: R Medicine
R Medicine > RK Dentistry
Divisions: 02. Fakultas Kedokteran Gigi > Oral Biology
Creators:
CreatorsNIM/NIDN
Retno Indrawati, NIDN. 0012115903UNSPECIFIED
Muhammad Lutfi, NIDN. 0006036704UNSPECIFIED
Erina Fatmala Yuli AndariUNSPECIFIED
Depositing User: Rudy Febiyanto
Date Deposited: 24 Jul 2019 00:48
Last Modified: 22 Oct 2019 06:33
URI: http://repository.unair.ac.id/id/eprint/85314
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