Efek Ekstrak Sambiloto (Andrographis Paniculata Nees) pada Ekspresi Telomerase dari Kanker Payudara Tikus yang Diinduksi dengan DMBA

Yurika Sastyarina and Junaidi Khotib, NIDN. 0022107001 and Sukardiman, NIDN. 0001096305 (2010) Efek Ekstrak Sambiloto (Andrographis Paniculata Nees) pada Ekspresi Telomerase dari Kanker Payudara Tikus yang Diinduksi dengan DMBA. Journal of Tropical Pharmacy and Chemistry, 1 (1). pp. 70-78. ISSN 2087-7099

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Abstract

It has been well documented that chemical carcinogen, 7.12 dimethylbenz-(a)anthracene (DMBA), plays a role in the incidence and growth of mammary cancer. Present study was designed to investigate the influence of Andrographis paniculata extract on telomerase activities on DMBA induced breast cancer in the female rat Sprague Dawley strain. DMBA-induced mammary cancer is a useful model to investigate the changes of epithelial cells that occur during mammary cancer progression. Mammary cancer model was induced 10 times twice a week by oral DMBA 20 mg/kg body weight. Mammary cancer occurred in 75 % animals nine weeks after oral administration of DMBA, it was represented with nodule on the mammary gland and the increasing of mammary gland volume compare with normal control F(1.8) = 731.711; p < 0.001. This study was also designed to investigate the effect of Andrographis paniculata extract mammary carcinoma induced by DMBA. Administration of three different dose of Andrographis paniculata (100 mg/kg, 300 mg/kg and 1000 mg/kg) had statistically different with mammary gland volume of DMBA treated rat F (4.17) = 92.777; p<0.05. So, Andrographis paniculata has significant effect on the treatment of DMBA-induced mammary carcinoma. The Epithelial cells were harvested on day 90 and stained with routine histology staining, hematoxylineosin, for morphological qualitative analysis, immunohistochemical examination. The lesions observed from the removed samples ranged widely from benign to malignant. The results showed that DMBA induce cell proliferation, nuclear irregularities, and numerous mitoses and induced cell necrosis. On immunohistochemical examination, it shows that Andrographis paniculata can stimulate of expression of p53 protein and increase the number of epithelial cells experience apoptosis.

Item Type: Article
Uncontrolled Keywords: Sambiloto (Andrographis Paniculata. Ness), DMBA, Kanker payudara, p53
Subjects: R Medicine
R Medicine > RS Pharmacy and materia medica
Divisions: 05. Fakultas Farmasi
Creators:
CreatorsNIM/NIDN
Yurika SastyarinaUNSPECIFIED
Junaidi Khotib, NIDN. 0022107001UNSPECIFIED
Sukardiman, NIDN. 0001096305UNSPECIFIED
Depositing User: Mr M. Fuad Sofyan
Date Deposited: 14 Aug 2019 08:51
Last Modified: 14 Aug 2019 08:51
URI: http://repository.unair.ac.id/id/eprint/85450
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