Effect of cellulose derivative matrix and oligosaccharide on the solid state and physical characteristics of dimethyldioctadecyl ammonium-liposomes for vaccine

Helmy Yusuf, NIDN. 0015077901 and Raditya Weka Nugraheni and Dwi Setyawan, NIDN. 0030117104 (2019) Effect of cellulose derivative matrix and oligosaccharide on the solid state and physical characteristics of dimethyldioctadecyl ammonium-liposomes for vaccine. Research in Pharmaceutical Sciences, 14 (1). pp. 1-11. ISSN 1735-9414

[img] Text (FULLTEXT)
C-03.pdf

Download (4MB)
[img] Text (SIMILARITY)
Effect of cellulose derivative matrix and oligosaccharide on the solid state and physical characteristics of dimethyldioctadecylammoniumliposomes for vaccine.pdf

Download (4MB)
[img] Text (PEER REVIEW)
C-03 Rev.pdf

Download (1MB)
Official URL: http://www.rpsjournal.net/article.asp?issn=1735-53...

Abstract

The present study was to investigate the effect of cellulose matrix and oligosaccharide on solid state and morphology characteristics of freeze-dried cationic dimethyldioctadecylammonium (DDA)-based liposomes encapsulating ovalbumin (OVA). The OVA-containing liposomes were protected using cellulose derivative matrix and oligosaccharide. Despite the fact that saccharides are known to preserve protein and lipid membranes during drying, however, collapse structure are often addressed. In other side, cellulose matrix potentially prevents collapsing as it has been widely used for matrix in drug delivery formulations to increase the mass for compact matrices of resultant products. Their solid state characteristics were determined in terms of their crystallinity using X-Ray diffraction (XRD), thermal properties and detection of phase separation using differential scanning calorimetry (DSC). Furthermore, their morphology was observed using scanning electron microscopy and transmission electron microscopy. The study revealed that formulation with either oligosaccharide and cellulose matrix demonstrated a miscible mixture with DDA and soy phosphatidylcholine (SPC) that might construct stable dried liposomal formulation. Phase separation was not observed in formula with combination of oligosaccharide and cellulose matrix where their DSC thermograms showed glass transition indicating amorphous structure and miscible mixture. XRD confirmed the absence of crystal-like properties, demonstrating prevented crystallization. The dry products were porous with spherical liposomes trapped in the matrices, signifying the ease in reconstitution. Furthermore, OVA were well-preserved as its recovery was more than 80%. The preservation of both liposomes and protein antigen were found to be dependent upon the incorporation of both oligosaccharide and cellulose matrix included in the formulation.

Item Type: Article
Uncontrolled Keywords: Cellulose matrix; Liposomes; Oligosaccharide; Ovalbumin; Vaccine.
Subjects: R Medicine
R Medicine > RS Pharmacy and materia medica
Divisions: 05. Fakultas Farmasi
Creators:
CreatorsNIM/NIDN
Helmy Yusuf, NIDN. 0015077901UNSPECIFIED
Raditya Weka NugraheniUNSPECIFIED
Dwi Setyawan, NIDN. 0030117104UNSPECIFIED
Depositing User: Mr M. Fuad Sofyan
Date Deposited: 19 Aug 2019 02:54
Last Modified: 19 Aug 2019 02:54
URI: http://repository.unair.ac.id/id/eprint/85757
Sosial Share:

Actions (login required)

View Item View Item