Development of A Method To Quantify The DNA Content in Cationic Peptide-DNA Nanoparticles

Arvind K. Jain and Helmy Yusuf, NIDN. 0015077901 and Aditya Pattani and Helen O. McCarthy and Denise M. McDonald and Vicky L. Kett (2014) Development of A Method To Quantify The DNA Content in Cationic Peptide-DNA Nanoparticles. Journal of Pharmaceutical and Biomedical Analysis, 100. pp. 236-242. ISSN 0731-7085

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Official URL: https://www.sciencedirect.com/science/article/pii/...

Abstract

Gene therapy has the potential to provide safe and targeted therapies for a variety of diseases. A range of intracellular gene delivery vehicles have been proposed for this purpose. Non-viral vectors are a particularly attractive option and among them cationic peptides have emerged as promising candidates. For the pharmaceutical formulation and application to clinical studies it is necessary to quantify the amount of pDNA condensed with the delivery system. There is a severe deficiency in this area, thus far no methods have been reported specifically for pDNA condensed with cationic peptide to form nanoparticles. The current study seeks to address this and describes the evaluation of a range of disruption agents to extract DNA from nanoparticles formed by condensation with cationic fusogenic peptides RALA and KALA. Only proteinase K exhibited efficient and reproducible results and compatibility with the PicoGreen reagent based quantification assay. Thus we report for the first time a simple and reliable method that can quantify the pDNA content in pDNA cationic peptide nanoparticles.

Item Type: Article
Uncontrolled Keywords: DNA quantificationCationic peptidePicoGreenProteinase KKALA nanoparticles
Subjects: R Medicine
R Medicine > RS Pharmacy and materia medica
Divisions: 05. Fakultas Farmasi
Creators:
CreatorsNIM/NIDN
Arvind K. JainUNSPECIFIED
Helmy Yusuf, NIDN. 0015077901UNSPECIFIED
Aditya PattaniUNSPECIFIED
Helen O. McCarthyUNSPECIFIED
Denise M. McDonaldUNSPECIFIED
Vicky L. KettUNSPECIFIED
Depositing User: Mr M. Fuad Sofyan
Date Deposited: 19 Aug 2019 02:50
Last Modified: 19 Aug 2019 02:50
URI: http://repository.unair.ac.id/id/eprint/85759
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