Development of polymeric–cationic peptide composite nanoparticles, a nanoparticle-in-nanoparticle system for controlled gene delivery

Arvind K. Jain and Ashley Massey and Helmy Yusuf, NIDN. 0015077901 and Denise M. McDonald and Helen O. McCarthy and Vicky L. Kett (2015) Development of polymeric–cationic peptide composite nanoparticles, a nanoparticle-in-nanoparticle system for controlled gene delivery. International Journal of Nanomedicine. pp. 7183-7196. ISSN 1178-2013

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Official URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC46645...

Abstract

We report the formulation of novel composite nanoparticles that combine the high transfection efficiency of cationic peptide-DNA nanoparticles with the biocompatibility and prolonged delivery of polylactic acid–polyethylene glycol (PLA-PEG). The cationic cell-penetrating peptide RALA was used to condense DNA into nanoparticles that were encapsulated within a range of PLA-PEG copolymers. The composite nanoparticles produced exhibited excellent physicochemical properties including size <200 nm and encapsulation efficiency >80%. Images of the composite nanoparticles obtained with a new transmission electron microscopy staining method revealed the peptide-DNA nanoparticles within the PLA-PEG matrix. Varying the copolymers modulated the DNA release rate >6 weeks in vitro. The best formulation was selected and was able to transfect cells while maintaining viability. The effect of transferrin-appended composite nanoparticles was also studied. Thus, we have demonstrated the manufacture of composite nanoparticles for the controlled delivery of DNA.

Item Type: Article
Uncontrolled Keywords: PLA-PEG, cationic peptide, gene delivery, composite nanoparticles, DNA, transfection
Subjects: R Medicine
R Medicine > RS Pharmacy and materia medica
Divisions: 05. Fakultas Farmasi
Creators:
CreatorsNIM/NIDN
Arvind K. JainUNSPECIFIED
Ashley MasseyUNSPECIFIED
Helmy Yusuf, NIDN. 0015077901UNSPECIFIED
Denise M. McDonaldUNSPECIFIED
Helen O. McCarthyUNSPECIFIED
Vicky L. KettUNSPECIFIED
Depositing User: Mr M. Fuad Sofyan
Date Deposited: 19 Aug 2019 02:43
Last Modified: 19 Aug 2019 02:43
URI: http://repository.unair.ac.id/id/eprint/85761
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