The Effect of N-Acetyl-L-Cysteine (NAC) on Plasma ADMA Level and The Expression of VCAM-1 Protein on Endotelial Dysfunction in Diabetic Rats.

Irma Santi and Budi Suprapti and Imam Susilo, NIDN. 0010086503 and Junaidi Khotib, NIDN. 0022107001 (2016) The Effect of N-Acetyl-L-Cysteine (NAC) on Plasma ADMA Level and The Expression of VCAM-1 Protein on Endotelial Dysfunction in Diabetic Rats. Buletin Penelitian Kesehatan, 44 (3). pp. 147-152. ISSN 0125-9695

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Abstract

Endothelial dysfunction is an early state of symptoms in a cardiovascular disease. An elevated oxidative stress plays a key role in the pathogenesis of macrovascular diabetic complication. The present study was design to evaluate the effect of NAC on plasma ADMA level and the expression of VCAM-1 protein on endothelial dysfunction in diabetic rats. Thirty male Sprague–Dawley rats were divided into 5 groups i.e. normal rats, diabetic rats, treatment with NAC 30 mg/kgBW, NAC 56 mg/kgBW and NAC 100 mg/kgBW. Diabetic rats model was induced by intraperitonial administration of alloxan monohydrate at dose of 150 mg/kgBW, diabetes occurred on 3nd day after alloxan injection and then started treatment of N-acetyl-L-cystein for 28 days. ADMA plasma level was analyzed with Elisa Reader and the expression of VCAM-1 protein was evaluated by immunohistochemistry. Conclusion of this research is that treatment with NAC 30 mg/kgBW, NAC 56 mg/kgBW and NAC 100 mg/kgBW for 28 days may prevent oxidative stress indicated by the decreasing of plasma ADMA level by 45.8%: 55.75% and 65.92%, respectively and the decreasing of the expression of VCAM-1 protein on endothelial dysfunction in diabetic rat by 24%: 31.75% and 58.92%, respectively.

Item Type: Article
Uncontrolled Keywords: (NAC), Endothelial dysfunction, ADMA, VCAM-1
Subjects: R Medicine > R Medicine (General)
R Medicine > RB Pathology
Divisions: 01. Fakultas Kedokteran > Patologi Anatomi
Creators:
CreatorsNIM/NIDN
Irma SantiUNSPECIFIED
Budi SupraptiUNSPECIFIED
Imam Susilo, NIDN. 0010086503imam-susilo@fk.unair.ac.id
Junaidi Khotib, NIDN. 0022107001UNSPECIFIED
Depositing User: hanif kurniawan
Date Deposited: 20 Aug 2019 03:49
Last Modified: 20 Aug 2019 03:49
URI: http://repository.unair.ac.id/id/eprint/85852
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