The Difference in Biofilm Molecular Weight in Streptococcus mutans and Aggregatibacter actinomycetemcomitans Induced by Sucrose and Soy Protein (Glycine Soja)

Indah Listiana Kriswandini and Markus Budi Rahardjo and Hendrik Setia Budi and Risma Amalia (2019) The Difference in Biofilm Molecular Weight in Streptococcus mutans and Aggregatibacter actinomycetemcomitans Induced by Sucrose and Soy Protein (Glycine Soja). Indian Journal of Dental Research, 30 (2). pp. 273-278. ISSN 19983603

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Abstract

Context: Biofilms consist of microbial cells and extracellular polymeric substance (EPS). Streptococcus mutans and Aggregatibacter actinomycetemcomtans are bacteria that can form biofilms and generate EPS. Biofilm formation can be induced by specific substances such as sucrose and protein. Aims: To identify the molecular weight that determines biofilm protein profile expression of S. mutans and A. actinomycetemcomitans induced by sucrose (carbohydrate) and soy protein (glycine soja). Settings and Design: Experimental laboratory study. Materials and Methods: Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS‑PAGE) was used to determine the molecular weight. Statistical Analysis Used: Nil. Results: The results of analysis of protein SDS‑PAGE showed the presence of 28 protein bands on A. actinomycetemcomitans biofilm in the media trypticase soy broth (TSB), 20 protein bands on biofilms of S. mutans in the media TSB, 29 protein bands on biofilm A. actinomycetemcomitans in the media brain heart infusion (BHI) + sucrose 2%, and 13 protein bands on biofilms of S. mutans in the media BHI + sucrose 2%. Conclusion: There are differences in biofilm protein profile expression that determine the molecular weight of S. mutans biofilm and A. actinomycetemcomitans induced by sucrose (carbohydrate) and soy protein (glycine soja).

Item Type: Article
Uncontrolled Keywords: Biofilm, molecular weight, protein
Subjects: R Medicine
R Medicine > RK Dentistry
Divisions: 02. Fakultas Kedokteran Gigi > Oral Biology
Creators:
CreatorsNIM/NIDN
Indah Listiana KriswandiniNIDN0030106303
Markus Budi RahardjoUNSPECIFIED
Hendrik Setia BudiNIDN0010067204
Risma AmaliaUNSPECIFIED
Depositing User: Rudy Febiyanto
Date Deposited: 28 Aug 2019 01:22
Last Modified: 23 Dec 2019 08:07
URI: http://repository.unair.ac.id/id/eprint/85924
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